Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 471309..471946 Replicon chromosome
Accession NZ_CP021976
Organism Bacillus velezensis strain T20E-257

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CEG11_RS02425 Protein ID WP_003156187.1
Coordinates 471596..471946 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CEG11_RS02420 Protein ID WP_003156188.1
Coordinates 471309..471590 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEG11_RS02400 467674..468273 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CEG11_RS02405 468366..468731 + 366 WP_003156192.1 holo-ACP synthase -
CEG11_RS02410 468896..469903 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
CEG11_RS02415 470020..471189 + 1170 WP_003156189.1 alanine racemase -
CEG11_RS02420 471309..471590 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CEG11_RS02425 471596..471946 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CEG11_RS02430 472064..472885 + 822 WP_003156182.1 STAS domain-containing protein -
CEG11_RS02435 472890..473255 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
CEG11_RS02440 473258..473659 + 402 WP_003156178.1 anti-sigma regulatory factor -
CEG11_RS02445 473671..474678 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
CEG11_RS02450 474742..475071 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CEG11_RS02455 475068..475550 + 483 WP_003156173.1 anti-sigma B factor RsbW -
CEG11_RS02460 475516..476304 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CEG11_RS02465 476304..476906 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T78050 WP_003156187.1 NZ_CP021976:471596-471946 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T78050 NZ_CP021976:471596-471946 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT78050 WP_003156188.1 NZ_CP021976:471309-471590 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT78050 NZ_CP021976:471309-471590 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References