Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3227541..3228132 Replicon chromosome
Accession NZ_CP021971
Organism Hafnia alvei strain CBA7135

Toxin (Protein)


Gene name Hha Uniprot ID A0A097R540
Locus tag CD201_RS14940 Protein ID WP_025799954.1
Coordinates 3227929..3228132 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A097R537
Locus tag CD201_RS14935 Protein ID WP_025799952.1
Coordinates 3227541..3227909 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CD201_RS14920 3223303..3226455 + 3153 WP_025799949.1 multidrug efflux RND transporter permease subunit -
CD201_RS14925 3226615..3226887 + 273 WP_043494989.1 type B 50S ribosomal protein L31 -
CD201_RS14930 3226887..3227027 + 141 WP_004091741.1 type B 50S ribosomal protein L36 -
CD201_RS14935 3227541..3227909 + 369 WP_025799952.1 Hha toxicity modulator TomB Antitoxin
CD201_RS14940 3227929..3228132 + 204 WP_025799954.1 hemolysin expression modulator Hha Toxin
CD201_RS14945 3228332..3228751 - 420 WP_111330886.1 DUF1311 domain-containing protein -
CD201_RS14955 3229237..3229575 + 339 WP_043494994.1 MGMT family protein -
CD201_RS14960 3229623..3230141 - 519 WP_025799960.1 YbaY family lipoprotein -
CD201_RS14965 3230434..3231294 + 861 WP_043494996.1 acyl-CoA thioesterase II -
CD201_RS14970 3231376..3232668 - 1293 WP_172625153.1 ammonium transporter AmtB -
CD201_RS14975 3232699..3233037 - 339 WP_004091732.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8133.40 Da        Isoelectric Point: 5.8423

>T78047 WP_025799954.1 NZ_CP021971:3227929-3228132 [Hafnia alvei]
MTKIDYLMRLRKCTTLETLERVIEKNKYELSDDELETFYSAADHRLAELTMNKLYDKIPAEVWKYVR

Download         Length: 204 bp

>T78047 NZ_CP021971:3227929-3228132 [Hafnia alvei]
ATGACTAAAATTGATTACCTTATGCGCTTACGAAAGTGTACAACGTTAGAAACTTTAGAGCGCGTAATTGAAAAAAACAA
ATATGAGCTATCCGACGATGAGCTAGAAACTTTCTACTCAGCCGCCGACCATCGTTTGGCCGAACTCACCATGAACAAAC
TGTATGATAAGATCCCTGCTGAAGTTTGGAAGTATGTTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14201.03 Da        Isoelectric Point: 4.7386

>AT78047 WP_025799952.1 NZ_CP021971:3227541-3227909 [Hafnia alvei]
MDEYTSYQHDITELKYLCNMLYNQGMEVLSDSHHGWVNDPTAVANLQLNELNEHIATFGLTFKIKYPKSSELTEILDEYL
DETYSLFSNYSINEPELKKWLKVKGRILRYLAGEKPASALNF

Download         Length: 369 bp

>AT78047 NZ_CP021971:3227541-3227909 [Hafnia alvei]
ATGGATGAGTACACGTCTTATCAGCACGATATAACTGAACTGAAATACCTGTGTAATATGCTGTATAATCAGGGAATGGA
AGTTCTGAGTGATAGCCATCATGGCTGGGTTAATGACCCAACTGCTGTAGCTAATCTGCAACTCAATGAATTAAACGAAC
ATATCGCAACGTTCGGTTTAACGTTCAAGATTAAGTATCCAAAGAGTTCAGAATTAACTGAAATATTAGATGAGTATCTT
GACGAAACTTACAGTCTGTTTAGTAATTACAGCATAAACGAACCAGAACTAAAAAAATGGCTTAAAGTAAAAGGCCGTAT
TTTGCGTTATTTAGCGGGTGAAAAACCAGCATCCGCGTTGAACTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A097R540

Antitoxin

Source ID Structure
AlphaFold DB A0A097R537

References