Detailed information of TA system TA078033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1360215..1361134
• Replicon: chromosome
• Accession: NZ_CP021970
• Organism: Bacillus licheniformis strain CBA7132

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: CD200_RS06995
• Protein ID: WP_003180879.1
• Coordinates: 1360388..1361134 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: CD200_RS06990
• Protein ID: WP_003180877.1
• Coordinates: 1360215..1360388 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD200_RS06970	1355640..1356737	+	1098	WP_003180867.1	mannonate dehydratase	-
CD200_RS06975	1356713..1357561	+	849	WP_009328728.1	SDR family oxidoreductase	-
CD200_RS06980	1357588..1358601	+	1014	WP_003180872.1	zinc-binding alcohol dehydrogenase family protein	-
CD200_RS06985	1358654..1359925	+	1272	WP_011197811.1	MFS transporter	-
CD200_RS06990	1360215..1360388	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CD200_RS06995	1360388..1361134	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CD200_RS07000	1361245..1362243	-	999	WP_009328723.1	inorganic phosphate transporter	-
CD200_RS07005	1362256..1362873	-	618	WP_003180884.1	DUF47 domain-containing protein	-
CD200_RS07010	1363205..1364962	+	1758	WP_061565952.1	gamma-glutamyltransferase	-
CD200_RS07015	1365091..1365735	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T78033 WP_003180879.1 NZ_CP021970:c1361134-1360388 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T78033 NZ_CP021970:c1361134-1360388 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAAGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT78033 WP_003180877.1 NZ_CP021970:c1360388-1360215 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT78033 NZ_CP021970:c1360388-1360215 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37