Detailed information of TA system TA078030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 2954..3864
• Replicon: plasmid tig00000006
• Accession: NZ_CP021961
• Organism: Klebsiella pneumoniae strain AR_0139

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AM386_RS29750
• Protein ID: WP_032413375.1
• Coordinates: 3394..3864 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: AM386_RS29745
• Protein ID: WP_032413443.1
• Coordinates: 2954..3397 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM386_RS29720	905..1252	+	348	WP_032413377.1	hypothetical protein	-
AM386_RS29725	1310..1537	+	228	Protein_2	transposase	-
AM386_RS29730	1738..2036	-	299	Protein_3	transposase	-
AM386_RS29735	2056..2241	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
AM386_RS29740	2342..2854	+	513	Protein_5	DUF4113 domain-containing protein	-
AM386_RS29745	2954..3397	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
AM386_RS29750	3394..3864	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
AM386_RS29755	3974..4234	-	261	WP_017901409.1	hypothetical protein	-
AM386_RS29760	4870..5838	+	969	WP_075209834.1	IS5-like element IS903B family transposase	-
AM386_RS31390	5894..6055	+	162	Protein_10	IS110 family transposase	-
AM386_RS29765	6072..6380	+	309	Protein_11	DDE-type integrase/transposase/recombinase	-
AM386_RS29770	6330..6500	-	171	WP_071571074.1	hypothetical protein	-
AM386_RS29775	6501..6881	+	381	Protein_13	EAL domain-containing protein	-
AM386_RS29780	7063..8067	-	1005	WP_000427620.1	IS110-like element IS4321 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VI / blaNDM-1 / tet(A) / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1	htpB	1..97389	97389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T78030 WP_032413375.1 NZ_CP021961:3394-3864 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T78030 NZ_CP021961:3394-3864 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATAGCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT78030 WP_032413443.1 NZ_CP021961:2954-3397 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT78030 NZ_CP021961:2954-3397 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTATGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCTCGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4