Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2802177..2802796 Replicon chromosome
Accession NZ_CP021960
Organism Klebsiella pneumoniae strain AR_0139

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM386_RS15465 Protein ID WP_002892050.1
Coordinates 2802177..2802395 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM386_RS15470 Protein ID WP_002892066.1
Coordinates 2802422..2802796 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM386_RS15435 2798224..2798487 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
AM386_RS15440 2798487..2798627 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AM386_RS15445 2798624..2799322 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
AM386_RS15450 2799423..2800874 + 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
AM386_RS15455 2800849..2801319 - 471 WP_002892026.1 YlaC family protein -
AM386_RS31680 2801340..2801480 + 141 WP_004147370.1 hypothetical protein -
AM386_RS15460 2801452..2802018 - 567 WP_002892030.1 maltose O-acetyltransferase -
AM386_RS15465 2802177..2802395 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM386_RS15470 2802422..2802796 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM386_RS15475 2803282..2806428 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
AM386_RS15480 2806451..2807644 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78018 WP_002892050.1 NZ_CP021960:c2802395-2802177 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T78018 NZ_CP021960:c2802395-2802177 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT78018 WP_002892066.1 NZ_CP021960:c2802796-2802422 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT78018 NZ_CP021960:c2802796-2802422 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References