Detailed information of TA system TA078016

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2160781..2161484
• Replicon: chromosome
• Accession: NZ_CP021960
• Organism: Klebsiella pneumoniae strain AR_0139

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: AM386_RS12250
• Protein ID: WP_065803899.1
• Coordinates: 2161143..2161484 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A377WBK0
• Locus tag: AM386_RS12245
• Protein ID: WP_053274373.1
• Coordinates: 2160781..2161122 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM386_RS12205	2156323..2157027	+	705	WP_065803893.1	WYL domain-containing protein	-
AM386_RS12210	2157027..2157596	+	570	WP_004929046.1	hypothetical protein	-
AM386_RS12215	2157633..2158085	+	453	WP_065803894.1	hypothetical protein	-
AM386_RS12220	2158082..2158531	+	450	WP_071571046.1	hypothetical protein	-
AM386_RS12225	2158603..2158824	+	222	WP_065803896.1	DUF905 domain-containing protein	-
AM386_RS12230	2158923..2159744	+	822	WP_065803897.1	DUF945 domain-containing protein	-
AM386_RS12235	2159775..2160215	+	441	WP_039567646.1	antirestriction protein	-
AM386_RS12240	2160228..2160770	+	543	WP_065803898.1	DNA repair protein RadC	-
AM386_RS12245	2160781..2161122	+	342	WP_053274373.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM386_RS12250	2161143..2161484	+	342	WP_065803899.1	TA system toxin CbtA family protein	Toxin
AM386_RS12260	2161739..2162746	+	1008	WP_004108748.1	restriction endonuclease	-
AM386_RS31130	2163114..2164225	+	1112	Protein_2054	IS3 family transposase	-
AM386_RS31135	2164242..2164430	+	189	Protein_2055	transposase	-
AM386_RS12295	2164629..2165513	-	885	WP_004192285.1	RES domain-containing protein	-
AM386_RS12300	2165524..2166196	-	673	Protein_2057	DUF4400 domain-containing protein	-
AM386_RS12305	2166196..2166462	-	267	WP_004178415.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2136563..2173027	36464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12756.74 Da
• Isoelectric Point: 9.0089

>T78016 WP_065803899.1 NZ_CP021960:2161143-2161484 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIHEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T78016 NZ_CP021960:2161143-2161484 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCATGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12773.41 Da
• Isoelectric Point: 6.6253

>AT78016 WP_053274373.1 NZ_CP021960:2160781-2161122 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT78016 NZ_CP021960:2160781-2161122 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A377WBK0