Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1600934..1601559 Replicon chromosome
Accession NZ_CP021960
Organism Klebsiella pneumoniae strain AR_0139

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag AM386_RS09505 Protein ID WP_004187928.1
Coordinates 1601176..1601559 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM386_RS09500 Protein ID WP_004150355.1
Coordinates 1600934..1601176 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM386_RS09475 1596936..1597535 + 600 WP_004151865.1 glucose-1-phosphatase -
AM386_RS09480 1597529..1598389 + 861 WP_004146232.1 virulence factor BrkB family protein -
AM386_RS09485 1598386..1598823 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM386_RS09490 1598868..1599809 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM386_RS09495 1599823..1600740 - 918 WP_004187929.1 alpha/beta hydrolase -
AM386_RS09500 1600934..1601176 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM386_RS09505 1601176..1601559 + 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
AM386_RS09510 1601733..1602662 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM386_RS09515 1602659..1603294 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM386_RS09520 1603291..1604193 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T78013 WP_004187928.1 NZ_CP021960:1601176-1601559 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T78013 NZ_CP021960:1601176-1601559 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT78013 WP_004150355.1 NZ_CP021960:1600934-1601176 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT78013 NZ_CP021960:1600934-1601176 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTACTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References