Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81732..82375 Replicon plasmid tig00000003
Accession NZ_CP021959
Organism Klebsiella pneumoniae strain AR_0139

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag AM386_RS01265 Protein ID WP_032425563.1
Coordinates 81732..82148 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag AM386_RS01270 Protein ID WP_016338373.1
Coordinates 82145..82375 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM386_RS01245 77659..78363 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM386_RS01250 78427..78822 + 396 Protein_92 IS6-like element IS26 family transposase -
AM386_RS31030 78846..78976 - 131 Protein_93 IS5/IS1182 family transposase -
AM386_RS01255 79010..80151 - 1142 Protein_94 HlyD family secretion protein -
AM386_RS01260 80813..81691 + 879 WP_009309916.1 restriction endonuclease -
AM386_RS01265 81732..82148 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
AM386_RS01270 82145..82375 - 231 WP_016338373.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM386_RS01285 82938..83156 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
AM386_RS01290 83158..83463 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
AM386_RS01295 83632..84027 + 396 WP_017899885.1 hypothetical protein -
AM386_RS01300 84054..84368 + 315 WP_053389906.1 hypothetical protein -
AM386_RS01305 84379..85395 + 1017 WP_017899884.1 hypothetical protein -
AM386_RS01310 85593..86387 + 795 WP_004197635.1 site-specific integrase -
AM386_RS31445 86819..87121 - 303 WP_071571079.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 - 1..89382 89382


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T78007 WP_032425563.1 NZ_CP021959:c82148-81732 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T78007 NZ_CP021959:c82148-81732 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT78007 WP_016338373.1 NZ_CP021959:c82375-82145 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT78007 NZ_CP021959:c82375-82145 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References