Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2068363..2068994 Replicon chromosome
Accession NZ_CP021955
Organism Klebsiella pneumoniae strain AR_0107

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag AM486_RS10480 Protein ID WP_012542177.1
Coordinates 2068363..2068539 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X1QFN9
Locus tag AM486_RS10485 Protein ID WP_012542176.1
Coordinates 2068587..2068994 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM486_RS10460 2063751..2064782 + 1032 WP_088296168.1 DUF968 domain-containing protein -
AM486_RS10465 2064795..2065136 + 342 WP_017898982.1 antitermination protein Q -
AM486_RS10470 2065161..2066195 - 1035 WP_088296426.1 DNA cytosine methyltransferase -
AM486_RS10475 2066215..2067969 - 1755 WP_163597061.1 ATP-binding protein -
AM486_RS10480 2068363..2068539 + 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
AM486_RS10485 2068587..2068994 + 408 WP_012542176.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AM486_RS10490 2069709..2069978 + 270 WP_032439907.1 holin -
AM486_RS10495 2069956..2070453 + 498 WP_088296428.1 lysozyme -
AM486_RS10500 2070450..2070800 + 351 WP_017898986.1 hypothetical protein -
AM486_RS10515 2072178..2072399 + 222 WP_032439852.1 hypothetical protein -
AM486_RS10520 2072553..2072915 + 363 WP_017898990.1 HNH endonuclease -
AM486_RS10525 2072867..2073184 + 318 WP_014228902.1 hypothetical protein -
AM486_RS10530 2073181..2073612 + 432 WP_077259664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2045851..2102130 56279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T77992 WP_012542177.1 NZ_CP021955:2068363-2068539 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T77992 NZ_CP021955:2068363-2068539 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14977.99 Da        Isoelectric Point: 4.4277

>AT77992 WP_012542176.1 NZ_CP021955:2068587-2068994 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT77992 NZ_CP021955:2068587-2068994 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1QFN9

References