Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1159618..1160237 Replicon chromosome
Accession NZ_CP021955
Organism Klebsiella pneumoniae strain AR_0107

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM486_RS05760 Protein ID WP_002892050.1
Coordinates 1159618..1159836 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag AM486_RS05765 Protein ID WP_002892066.1
Coordinates 1159863..1160237 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM486_RS05730 1155665..1155928 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
AM486_RS05735 1155928..1156068 + 141 WP_040219999.1 type B 50S ribosomal protein L36 -
AM486_RS05740 1156065..1156763 - 699 WP_040220001.1 GNAT family N-acetyltransferase -
AM486_RS05745 1156864..1158315 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
AM486_RS05750 1158290..1158760 - 471 WP_002892026.1 YlaC family protein -
AM486_RS29390 1158781..1158921 + 141 WP_004147370.1 hypothetical protein -
AM486_RS05755 1158893..1159459 - 567 WP_002892030.1 maltose O-acetyltransferase -
AM486_RS05760 1159618..1159836 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM486_RS05765 1159863..1160237 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
AM486_RS05770 1160723..1163869 - 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit -
AM486_RS05775 1163892..1165085 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T77991 WP_002892050.1 NZ_CP021955:c1159836-1159618 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T77991 NZ_CP021955:c1159836-1159618 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT77991 WP_002892066.1 NZ_CP021955:c1160237-1159863 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT77991 NZ_CP021955:c1160237-1159863 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References