Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 83148..83884 Replicon plasmid tig00000168_pilon
Accession NZ_CP021951
Organism Klebsiella pneumoniae strain AR_0148

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM395_RS27205 Protein ID WP_003026803.1
Coordinates 83148..83630 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM395_RS27210 Protein ID WP_003026799.1
Coordinates 83618..83884 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM395_RS27185 78502..80265 - 1764 WP_004200842.1 DUF262 domain-containing protein -
AM395_RS27190 80463..80669 - 207 Protein_67 helix-turn-helix domain-containing protein -
AM395_RS27195 80883..81515 + 633 WP_001567369.1 hypothetical protein -
AM395_RS27200 81544..82947 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM395_RS27205 83148..83630 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM395_RS27210 83618..83884 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM395_RS27215 84129..84563 - 435 WP_001567367.1 cell envelope integrity protein TolA -
AM395_RS27220 84610..85158 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
AM395_RS27230 85652..85960 + 309 WP_017896554.1 hypothetical protein -
AM395_RS27245 86859..88397 - 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
AM395_RS27250 88446..88793 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / tet(A) - 1..181589 181589
- inside IScluster/Tn - - 81544..89194 7650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T77985 WP_003026803.1 NZ_CP021951:c83630-83148 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T77985 NZ_CP021951:c83630-83148 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT77985 WP_003026799.1 NZ_CP021951:c83884-83618 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT77985 NZ_CP021951:c83884-83618 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References