Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11370..12013 Replicon plasmid tig00000200
Accession NZ_CP021947
Organism Klebsiella pneumoniae strain AR_0152

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag AM399_RS28275 Protein ID WP_000754566.1
Coordinates 11370..11786 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AM399_RS28280 Protein ID WP_001261276.1
Coordinates 11783..12013 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM399_RS29450 6644..6754 + 111 Protein_9 Rpn family recombination-promoting nuclease/putative transposase -
AM399_RS28240 6751..7362 - 612 WP_000176305.1 DUF2913 family protein -
AM399_RS28245 7416..7697 - 282 WP_001568067.1 hypothetical protein -
AM399_RS28250 7870..8205 + 336 WP_011977766.1 C2H2-type zinc finger protein -
AM399_RS29455 8831..8944 + 114 Protein_13 Tn3 family transposase -
AM399_RS28260 8952..10028 - 1077 WP_000227969.1 IS110 family transposase -
AM399_RS28270 10310..11166 - 857 Protein_15 IS3-like element ISEc15 family transposase -
AM399_RS28275 11370..11786 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
AM399_RS28280 11783..12013 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM399_RS28295 12930..13238 + 309 WP_004152336.1 hypothetical protein -
AM399_RS28300 13266..13595 + 330 WP_004152337.1 hypothetical protein -
AM399_RS28305 13621..14019 + 399 WP_004171440.1 hypothetical protein -
AM399_RS28310 14026..14358 + 333 WP_004152339.1 hypothetical protein -
AM399_RS28315 14358..15140 + 783 WP_004152340.1 site-specific integrase -
AM399_RS28325 16354..16827 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / blaCTX-M-15 / qnrS1 - 1..54064 54064
- flank IS/Tn - - 16947..18215 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T77966 WP_000754566.1 NZ_CP021947:c11786-11370 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T77966 NZ_CP021947:c11786-11370 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT77966 WP_001261276.1 NZ_CP021947:c12013-11783 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT77966 NZ_CP021947:c12013-11783 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References