Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2351553..2352178 Replicon chromosome
Accession NZ_CP021944
Organism Klebsiella pneumoniae strain AR_0152

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AM399_RS11735 Protein ID WP_002882817.1
Coordinates 2351795..2352178 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM399_RS11730 Protein ID WP_004150355.1
Coordinates 2351553..2351795 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM399_RS11705 2347544..2348143 + 600 WP_004151865.1 glucose-1-phosphatase -
AM399_RS11710 2348137..2348997 + 861 WP_004146232.1 virulence factor BrkB family protein -
AM399_RS11715 2348994..2349431 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM399_RS11720 2349476..2350417 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM399_RS11725 2350431..2351348 - 918 WP_021466676.1 alpha/beta hydrolase -
AM399_RS11730 2351553..2351795 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM399_RS11735 2351795..2352178 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AM399_RS11740 2352352..2353281 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM399_RS11745 2353278..2353913 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM399_RS11750 2353910..2354812 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T77960 WP_002882817.1 NZ_CP021944:2351795-2352178 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T77960 NZ_CP021944:2351795-2352178 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT77960 WP_004150355.1 NZ_CP021944:2351553-2351795 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT77960 NZ_CP021944:2351553-2351795 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References