Detailed information of TA system TA077946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9131..9774
• Replicon: plasmid tig00000217
• Accession: NZ_CP021941
• Organism: Klebsiella pneumoniae strain AR_0145

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: AM392_RS27415
• Protein ID: WP_000754566.1
• Coordinates: 9358..9774 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: AM392_RS27410
• Protein ID: WP_001261276.1
• Coordinates: 9131..9361 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM392_RS27365	4317..4790	+	474	WP_004152341.1	YkgJ family cysteine cluster protein	-
AM392_RS27370	4882..5112	+	231	WP_011977773.1	hypothetical protein	-
AM392_RS27375	6004..6786	-	783	WP_004152340.1	site-specific integrase	-
AM392_RS27380	6786..7118	-	333	WP_004152339.1	hypothetical protein	-
AM392_RS27385	7125..7523	-	399	WP_004171440.1	hypothetical protein	-
AM392_RS27390	7549..7878	-	330	WP_004152337.1	hypothetical protein	-
AM392_RS27395	7906..8214	-	309	WP_004152336.1	hypothetical protein	-
AM392_RS27410	9131..9361	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM392_RS27415	9358..9774	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM392_RS27420	9978..10834	+	857	Protein_11	IS3-like element ISEc15 family transposase	-
AM392_RS27430	11116..12192	+	1077	WP_000227969.1	IS110 family transposase	-
AM392_RS28585	12200..12313	-	114	Protein_13	Tn3 family transposase	-
AM392_RS27440	12939..13274	-	336	WP_011977766.1	C2H2-type zinc finger protein	-
AM392_RS27445	13447..13728	+	282	WP_001568067.1	hypothetical protein	-
AM392_RS27450	13782..14393	+	612	WP_000176305.1	DUF2913 family protein	-
AM392_RS28590	14390..14500	-	111	Protein_17	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / qnrS1 / blaCTX-M-15 / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..54064	54064
	-	flank	IS/Tn	-	-	2929..4197	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T77946 WP_000754566.1 NZ_CP021941:9358-9774 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T77946 NZ_CP021941:9358-9774 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT77946 WP_001261276.1 NZ_CP021941:9131-9361 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT77946 NZ_CP021941:9131-9361 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023