Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4639239..4639975 Replicon chromosome
Accession NZ_CP021939
Organism Klebsiella pneumoniae strain AR_0145

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag AM392_RS23095 Protein ID WP_032433360.1
Coordinates 4639239..4639721 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM392_RS23100 Protein ID WP_003026799.1
Coordinates 4639709..4639975 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM392_RS23070 4634249..4634827 + 579 WP_032432061.1 type 1 fimbrial protein -
AM392_RS23075 4634839..4635783 + 945 WP_077254249.1 fimbrial protein -
AM392_RS23080 4635996..4636595 + 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
AM392_RS23085 4636975..4637607 + 633 WP_001567369.1 hypothetical protein -
AM392_RS23090 4637636..4639039 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM392_RS23095 4639239..4639721 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
AM392_RS23100 4639709..4639975 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM392_RS23105 4640288..4640851 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
AM392_RS23110 4640861..4641493 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
AM392_RS23115 4641511..4642515 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
AM392_RS23120 4642499..4643161 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
AM392_RS23125 4643190..4644329 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4637636..4639039 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T77943 WP_032433360.1 NZ_CP021939:c4639721-4639239 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T77943 NZ_CP021939:c4639721-4639239 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT77943 WP_003026799.1 NZ_CP021939:c4639975-4639709 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT77943 NZ_CP021939:c4639975-4639709 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References