Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 823236..823861 Replicon chromosome
Accession NZ_CP021939
Organism Klebsiella pneumoniae strain AR_0145

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag AM392_RS04205 Protein ID WP_002882817.1
Coordinates 823478..823861 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM392_RS04200 Protein ID WP_004150355.1
Coordinates 823236..823478 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM392_RS04175 819227..819826 + 600 WP_004151865.1 glucose-1-phosphatase -
AM392_RS04180 819820..820680 + 861 WP_004146232.1 virulence factor BrkB family protein -
AM392_RS04185 820677..821114 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM392_RS04190 821159..822100 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM392_RS04195 822114..823031 - 918 WP_021466676.1 alpha/beta hydrolase -
AM392_RS04200 823236..823478 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM392_RS04205 823478..823861 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
AM392_RS04210 824035..824964 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM392_RS04215 824961..825596 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
AM392_RS04220 825593..826495 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T77929 WP_002882817.1 NZ_CP021939:823478-823861 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T77929 NZ_CP021939:823478-823861 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT77929 WP_004150355.1 NZ_CP021939:823236-823478 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT77929 NZ_CP021939:823236-823478 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References