Detailed information of TA system TA077918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 23306..23853
• Replicon: plasmid unitig_4
• Accession: NZ_CP021938
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AM434_RS28910
• Protein ID: WP_000970000.1
• Coordinates: 23306..23587 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: AM434_RS28915
• Protein ID: WP_000079928.1
• Coordinates: 23584..23853 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS28865	18449..18667	-	219	WP_032256270.1	type II toxin-antitoxin system antitoxin CcdA	-
AM434_RS28870	19215..20084	+	870	WP_032256267.1	hypothetical protein	-
AM434_RS28875	20147..20332	-	186	WP_032256266.1	hypothetical protein	-
AM434_RS28885	20556..21146	+	591	WP_032256264.1	hypothetical protein	-
AM434_RS28890	21164..21511	-	348	WP_088251382.1	hypothetical protein	-
AM434_RS28900	21813..22295	-	483	WP_001311056.1	hypothetical protein	-
AM434_RS28905	22412..23260	-	849	WP_032256585.1	3'-5' exonuclease	-
AM434_RS28910	23306..23587	-	282	WP_000970000.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM434_RS28915	23584..23853	-	270	WP_000079928.1	hypothetical protein	Antitoxin
AM434_RS28930	24768..25625	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
AM434_RS28935	25618..25692	-	75	WP_001365705.1	RepA leader peptide Tap	-
AM434_RS28945	25937..26185	-	249	WP_000083839.1	replication regulatory protein RepA	-
AM434_RS28950	26470..26619	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
-	26664..26723	+	60	NuclAT_1	-	-
-	26664..26723	+	60	NuclAT_1	-	-
-	26664..26723	+	60	NuclAT_1	-	-
-	26664..26723	+	60	NuclAT_1	-	-
AM434_RS28960	26866..27339	-	474	WP_016240489.1	hypothetical protein	-
AM434_RS28965	27494..28084	-	591	WP_042018152.1	DUF2726 domain-containing protein	-
AM434_RS28970	28122..28331	-	210	WP_001369435.1	hemolysin expression modulator Hha	-
AM434_RS28975	28377..28838	-	462	WP_016230918.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..75318	75318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10921.62 Da
• Isoelectric Point: 8.4615

>T77918 WP_000970000.1 NZ_CP021938:c23587-23306 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLVGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T77918 NZ_CP021938:c23587-23306 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGTCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT77918 WP_000079928.1 NZ_CP021938:c23853-23584 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT77918 NZ_CP021938:c23853-23584 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5W2J4D0