77917

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 18142..18667 Replicon plasmid unitig_4
Accession NZ_CP021938
Organism Escherichia coli strain AR_0055

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AM434_RS28860 Protein ID WP_001159871.1
Coordinates 18142..18447 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A4D9F4P3
Locus tag AM434_RS28865 Protein ID WP_032256270.1
Coordinates 18449..18667 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM434_RS28805 13562..14089 + 528 WP_001196880.1 DoxX family protein -
AM434_RS28810 14422..14784 - 363 WP_000181069.1 hypothetical protein -
AM434_RS28815 14781..14909 - 129 Protein_22 DUF2164 family protein -
AM434_RS28825 15169..15270 - 102 Protein_23 methionyl-tRNA formyltransferase -
AM434_RS28830 15285..15794 - 510 WP_000115001.1 peptide deformylase -
AM434_RS28840 16322..16723 + 402 WP_050488175.1 hypothetical protein -
AM434_RS28845 16686..17820 + 1135 Protein_26 IS91 family transposase -
AM434_RS28860 18142..18447 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AM434_RS28865 18449..18667 - 219 WP_032256270.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM434_RS28870 19215..20084 + 870 WP_032256267.1 hypothetical protein -
AM434_RS28875 20147..20332 - 186 WP_032256266.1 hypothetical protein -
AM434_RS28885 20556..21146 + 591 WP_032256264.1 hypothetical protein -
AM434_RS28890 21164..21511 - 348 WP_088251382.1 hypothetical protein -
AM434_RS28900 21813..22295 - 483 WP_001311056.1 hypothetical protein -
AM434_RS28905 22412..23260 - 849 WP_032256585.1 3'-5' exonuclease -
AM434_RS28910 23306..23587 - 282 WP_000970000.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..75318 75318
- flank IS/Tn - - 16966..17820 854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T77917 WP_001159871.1 NZ_CP021938:c18447-18142 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77917 NZ_CP021938:c18447-18142 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8388.34 Da        Isoelectric Point: 4.7405

>AT77917 WP_032256270.1 NZ_CP021938:c18667-18449 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMSEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77917 NZ_CP021938:c18667-18449 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGTCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A4D9F4P3

References