Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 69256..69781 Replicon plasmid unitig_3j3rc_linear
Accession NZ_CP021937
Organism Escherichia coli strain AR_0055

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AM434_RS28105 Protein ID WP_001159868.1
Coordinates 69476..69781 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AM434_RS28100 Protein ID WP_000813634.1
Coordinates 69256..69474 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM434_RS28075 64535..66148 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
AM434_RS28080 66179..66529 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
AM434_RS28085 66526..66951 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
AM434_RS28090 67013..68164 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
AM434_RS28095 68198..68710 - 513 WP_000151784.1 hypothetical protein -
AM434_RS28100 69256..69474 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM434_RS28105 69476..69781 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AM434_RS28110 69782..70588 + 807 WP_000016982.1 site-specific integrase -
AM434_RS28115 71362..72117 + 756 WP_000852146.1 replication initiation protein RepE -
AM434_RS28120 72705..73871 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / aac(3)-IIa / tet(A) / aac(6')-Ib-cr / blaOXA-1 - 1..151110 151110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T77916 WP_001159868.1 NZ_CP021937:69476-69781 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77916 NZ_CP021937:69476-69781 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT77916 WP_000813634.1 NZ_CP021937:69256-69474 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77916 NZ_CP021937:69256-69474 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References