Detailed information of TA system TA077913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 50257..50816
• Replicon: plasmid unitig_3j3rc_linear
• Accession: NZ_CP021937
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: AM434_RS27985
• Protein ID: WP_000421272.1
• Coordinates: 50541..50816 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: AM434_RS27980
• Protein ID: WP_001387467.1
• Coordinates: 50257..50541 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS27950	45720..46160	-	441	Protein_57	zinc-binding dehydrogenase	-
AM434_RS27955	46134..46328	+	195	WP_000796505.1	hypothetical protein	-
AM434_RS27960	46561..47451	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
AM434_RS27965	47476..47856	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
AM434_RS27970	47889..48854	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
AM434_RS27975	48900..49652	-	753	WP_000562172.1	IclR family transcriptional regulator	-
AM434_RS27980	50257..50541	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM434_RS27985	50541..50816	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM434_RS27990	50922..51215	+	294	WP_001105060.1	hypothetical protein	-
AM434_RS29670	51250..51378	-	129	Protein_66	IS481 family transposase	-
AM434_RS27995	51411..52580	-	1170	WP_001072355.1	hypothetical protein	-
AM434_RS28005	53073..53380	+	308	Protein_68	type II toxin-antitoxin system ParD family antitoxin	-
AM434_RS28020	53669..54366	+	698	Protein_69	IS1 family transposase	-
AM434_RS28030	54620..55642	-	1023	WP_000361402.1	helicase UvrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA5 / aac(3)-IIa / tet(A) / aac(6')-Ib-cr / blaOXA-1	-	1..151110	151110
	-	flank	IS/Tn	-	-	53989..54366	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T77913 WP_000421272.1 NZ_CP021937:50541-50816 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T77913 NZ_CP021937:50541-50816 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT77913 WP_001387467.1 NZ_CP021937:50257-50541 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT77913 NZ_CP021937:50257-50541 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880