77903

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3224701..3225221 Replicon chromosome
Accession NZ_CP021935
Organism Escherichia coli strain AR_0055

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag AM434_RS16570 Protein ID WP_001160964.1
Coordinates 3224937..3225221 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AM434_RS16565 Protein ID WP_000125566.1
Coordinates 3224701..3224934 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM434_RS16540 3219774..3220061 + 288 WP_000203744.1 ferredoxin-like protein FixX -
AM434_RS16545 3220120..3221451 + 1332 WP_001183209.1 MFS transporter -
AM434_RS16550 3221559..3222089 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AM434_RS16555 3222082..3223944 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
AM434_RS16560 3224136..3224615 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
AM434_RS16565 3224701..3224934 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM434_RS16570 3224937..3225221 + 285 WP_001160964.1 CcdB family protein Toxin
AM434_RS16575 3225248..3226096 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AM434_RS16580 3226103..3226480 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AM434_RS16585 3226483..3227304 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AM434_RS16590 3227301..3228290 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AM434_RS16595 3228290..3229576 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T77903 WP_001160964.1 NZ_CP021935:3224937-3225221 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T77903 NZ_CP021935:3224937-3225221 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT77903 WP_000125566.1 NZ_CP021935:3224701-3224934 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT77903 NZ_CP021935:3224701-3224934 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References