Detailed information of TA system TA077900

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2994540..2995375
• Replicon: chromosome
• Accession: NZ_CP021935
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: AM434_RS15285
• Protein ID: WP_000854759.1
• Coordinates: 2994998..2995375 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: AM434_RS15280
• Protein ID: WP_001295723.1
• Coordinates: 2994540..2994908 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS15240	2991658..2991816	+	159	WP_001323397.1	DUF905 family protein	-
AM434_RS15250	2991971..2992789	+	819	WP_088251357.1	DUF945 domain-containing protein	-
AM434_RS15255	2992789..2993034	+	246	WP_001164966.1	hypothetical protein	-
AM434_RS15260	2993128..2993601	+	474	WP_001542276.1	antirestriction protein	-
AM434_RS15265	2993617..2994093	+	477	WP_001186775.1	RadC family protein	-
AM434_RS15270	2994156..2994377	+	222	WP_000692345.1	DUF987 domain-containing protein	-
AM434_RS15280	2994540..2994908	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM434_RS15285	2994998..2995375	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
AM434_RS15290	2995372..2995860	+	489	WP_000761690.1	hypothetical protein	-
AM434_RS15295	2995877..2996053	+	177	WP_000839286.1	DUF957 domain-containing protein	-
AM434_RS29470	2996153..2996311	+	159	WP_001467148.1	hypothetical protein	-
AM434_RS29480	2996675..2996851	+	177	Protein_2792	helix-turn-helix domain-containing protein	-
AM434_RS15310	2997624..2999264	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T77900 WP_000854759.1 NZ_CP021935:2994998-2995375 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T77900 NZ_CP021935:2994998-2995375 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT77900 WP_001295723.1 NZ_CP021935:2994540-2994908 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT77900 NZ_CP021935:2994540-2994908 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52