Detailed information of TA system TA077898

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2738116..2738951
• Replicon: chromosome
• Accession: NZ_CP021935
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A5F0P695
• Locus tag: AM434_RS13800
• Protein ID: WP_022645116.1
• Coordinates: 2738116..2738493 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: AM434_RS13805
• Protein ID: WP_001295723.1
• Coordinates: 2738583..2738951 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS13780	2734483..2736021	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
AM434_RS13790	2736770..2737612	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
AM434_RS13795	2737697..2737891	-	195	WP_024181941.1	DUF957 domain-containing protein	-
AM434_RS29430	2737970..2738119	-	150	Protein_2526	hypothetical protein	-
AM434_RS13800	2738116..2738493	-	378	WP_022645116.1	TA system toxin CbtA family protein	Toxin
AM434_RS13805	2738583..2738951	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM434_RS13815	2739114..2739335	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM434_RS13820	2739398..2739874	-	477	WP_022645117.1	RadC family protein	-
AM434_RS13825	2739890..2740369	-	480	WP_001564060.1	antirestriction protein	-
AM434_RS13830	2740635..2741453	-	819	WP_001175165.1	DUF945 domain-containing protein	-
AM434_RS13835	2741543..2741776	-	234	WP_022645118.1	DUF905 family protein	-
AM434_RS13840	2741782..2742459	-	678	WP_001097302.1	hypothetical protein	-
AM434_RS13845	2742607..2743287	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2711079..2772716	61637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14021.99 Da
• Isoelectric Point: 7.8045

>T77898 WP_022645116.1 NZ_CP021935:c2738493-2738116 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T77898 NZ_CP021935:c2738493-2738116 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGTTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT77898 WP_001295723.1 NZ_CP021935:c2738951-2738583 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT77898 NZ_CP021935:c2738951-2738583 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A5F0P695

Antitoxin

• AlphaFold DB: S1NM52