Detailed information of TA system TA077892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 1379976..1381257
• Replicon: chromosome
• Accession: NZ_CP021935
• Organism: Escherichia coli strain AR_0055

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: AM434_RS07000
• Protein ID: WP_088251337.1
• Coordinates: 1380364..1381257 (+)
• Length: 298 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: S1P7P2
• Locus tag: AM434_RS06995
• Protein ID: WP_000208382.1
• Coordinates: 1379976..1380371 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM434_RS06950	1375470..1375955	+	486	WP_000849565.1	antirestriction protein	-
AM434_RS06955	1375971..1376447	+	477	WP_001186726.1	RadC family protein	-
AM434_RS06960	1376510..1376731	+	222	WP_000692329.1	DUF987 domain-containing protein	-
AM434_RS06965	1376750..1377394	+	645	WP_000094916.1	hypothetical protein	-
AM434_RS06970	1377444..1377812	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	-
AM434_RS06975	1377901..1378278	+	378	WP_000854690.1	TA system toxin CbtA family protein	-
AM434_RS06980	1378275..1378763	+	489	WP_000761699.1	hypothetical protein	-
AM434_RS06985	1378780..1378977	+	198	WP_000839293.1	DUF957 domain-containing protein	-
AM434_RS06990	1379062..1379907	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
AM434_RS06995	1379976..1380371	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
AM434_RS07000	1380364..1381257	+	894	WP_088251337.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
AM434_RS07005	1381728..1381898	+	171	Protein_1291	IS110 family transposase	-
AM434_RS07010	1382247..1383746	+	1500	WP_000174401.1	IS21-like element ISEc10 family transposase	-
AM434_RS07015	1383743..1384498	+	756	Protein_1293	IS21-like element ISEc10 family helper ATPase IstB	-
AM434_RS07020	1385125..1386108	+	984	WP_140423830.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1335706..1381898	46192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-292)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 298 a.a.
• Molecular weight: 34254.35 Da
• Isoelectric Point: 5.9850

>T77892 WP_088251337.1 NZ_CP021935:1380364-1381257 [Escherichia coli]
MPELTSLFPDVADALDIESVAIVEKDYFVVDLLRLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKEIVQIITDVIHNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPVTAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDYPVHKLRFQRFVNPMVFGNTQIN

Nucleotide

• Length: 894 bp

>T77892 NZ_CP021935:1380364-1381257 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTGTAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14640.09 Da
• Isoelectric Point: 11.3150

>AT77892 WP_000208382.1 NZ_CP021935:1379976-1380371 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITE
ALERLNIRYRLAGATECYNKGQSTQIPVSLGIKTPSRFKRMLIVGKSKINA

Nucleotide

• Length: 396 bp

>AT77892 NZ_CP021935:1379976-1380371 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAATGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA

Structures

Antitoxin

• AlphaFold DB: S1P7P2