Detailed information of TA system TA077878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 81429..82014
• Replicon: plasmid pAP1468-2
• Accession: NZ_CP021924
• Organism: Acetobacter pasteurianus subsp. Pasteurianus strain SRCM101468

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C7JIK9
• Locus tag: S101468_RS15525
• Protein ID: WP_012813213.1
• Coordinates: 81685..82014 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: C7JIL0
• Locus tag: S101468_RS15520
• Protein ID: WP_006115554.1
• Coordinates: 81429..81683 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101468_RS15485	76511..77530	-	1020	WP_006115550.1	cytochrome d ubiquinol oxidase subunit II	-
S101468_RS15490	77533..78966	-	1434	WP_006115551.1	cytochrome ubiquinol oxidase subunit I	-
S101468_RS15500	79402..80362	-	961	Protein_70	IS481 family transposase	-
S101468_RS15505	80420..80680	+	261	Protein_71	hypothetical protein	-
S101468_RS15510	80753..81025	+	273	WP_006115552.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
S101468_RS15515	81022..81330	+	309	WP_006115553.1	PIN domain-containing protein	-
S101468_RS15520	81429..81683	+	255	WP_006115554.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
S101468_RS15525	81685..82014	+	330	WP_012813213.1	endoribonuclease MazF	Toxin
S101468_RS15530	82071..82271	+	201	WP_012813212.1	hypothetical protein	-
S101468_RS15535	82301..82621	+	321	WP_012813211.1	hypothetical protein	-
S101468_RS15540	82678..83082	-	405	WP_006115557.1	hypothetical protein	-
S101468_RS15545	83095..83910	-	816	WP_012813210.1	hypothetical protein	-
S101468_RS16770	83995..84180	+	186	WP_041633309.1	hypothetical protein	-
S101468_RS15555	84232..85415	+	1184	WP_088365127.1	IS3 family transposase	-
S101468_RS15560	85622..85900	+	279	WP_006115563.1	HU family DNA-binding protein	-
S101468_RS15565	85979..86197	+	219	WP_170315285.1	WGR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..184348	184348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 12.8374

>T77878 WP_012813213.1 NZ_CP021924:81685-82014 [Acetobacter pasteurianus subsp. Pasteurianus]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 330 bp

>T77878 NZ_CP021924:81685-82014 [Acetobacter pasteurianus subsp. Pasteurianus]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 10912.81 Da
• Isoelectric Point: 12.7242

>AT77878 WP_006115554.1 NZ_CP021924:81429-81683 [Acetobacter pasteurianus subsp. Pasteurianus]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKK

Nucleotide

• Length: 255 bp

>AT77878 NZ_CP021924:81429-81683 [Acetobacter pasteurianus subsp. Pasteurianus]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: A0A6P1QFJ7

Antitoxin

• AlphaFold DB: A0A6P1QDC4