Detailed information of TA system TA077874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 529105..529741
• Replicon: chromosome
• Accession: NZ_CP021921
• Organism: Bacillus subtilis subsp. subtilis strain SRCM101392

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: S101392_RS02750
• Protein ID: WP_003156187.1
• Coordinates: 529391..529741 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: S101392_RS02745
• Protein ID: WP_003225183.1
• Coordinates: 529105..529386 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S101392_RS02725	525464..526063	-	600	WP_088325331.1	rhomboid family intramembrane serine protease	-
S101392_RS02730	526158..526523	+	366	WP_088325332.1	holo-ACP synthase	-
S101392_RS02735	526689..527705	+	1017	WP_088327413.1	outer membrane lipoprotein carrier protein LolA	-
S101392_RS02740	527820..528989	+	1170	WP_015252766.1	alanine racemase	-
S101392_RS02745	529105..529386	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
S101392_RS02750	529391..529741	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
S101392_RS02755	529856..530680	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
S101392_RS02760	530685..531050	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
S101392_RS02765	531054..531455	+	402	WP_017697001.1	serine/threonine-protein kinase RsbT	-
S101392_RS02770	531467..532474	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
S101392_RS02775	532536..532865	+	330	WP_080528943.1	anti-sigma factor antagonist RsbV	-
S101392_RS02780	532862..533344	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
S101392_RS02785	533310..534098	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
S101392_RS02790	534098..534697	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T77874 WP_003156187.1 NZ_CP021921:529391-529741 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T77874 NZ_CP021921:529391-529741 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT77874 WP_003225183.1 NZ_CP021921:529105-529386 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT77874 NZ_CP021921:529105-529386 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32