Detailed information of TA system TA077862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1199698..1200614
• Replicon: chromosome
• Accession: NZ_CP021911
• Organism: Bacillus sp. MD-5

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CDO84_RS06015
• Protein ID: WP_040083357.1
• Coordinates: 1199868..1200614 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4NV07
• Locus tag: CDO84_RS06010
• Protein ID: WP_003239095.1
• Coordinates: 1199698..1199868 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO84_RS05980	1196559..1196888	+	330	WP_040083361.1	XkdW family protein	-
CDO84_RS05985	1196885..1197049	+	165	WP_040083360.1	XkdX family protein	-
CDO84_RS05990	1197094..1197933	+	840	WP_040083359.1	hypothetical protein	-
CDO84_RS05995	1197986..1198255	+	270	WP_003232655.1	hemolysin XhlA family protein	-
CDO84_RS06000	1198268..1198531	+	264	WP_003232653.1	phage holin	-
CDO84_RS06005	1198544..1199437	+	894	WP_040083358.1	N-acetylmuramoyl-L-alanine amidase	-
CDO84_RS21340	1199475..1199612	-	138	WP_136654612.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
CDO84_RS06010	1199698..1199868	-	171	WP_003239095.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
CDO84_RS06015	1199868..1200614	-	747	WP_040083357.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
CDO84_RS06020	1200724..1201725	-	1002	WP_014663667.1	inorganic phosphate transporter family protein	-
CDO84_RS06025	1201738..1202355	-	618	WP_040083356.1	DUF47 domain-containing protein	-
CDO84_RS06030	1202632..1203948	-	1317	WP_040083355.1	serine/threonine exchanger	-
CDO84_RS06035	1204334..1205284	+	951	WP_040083354.1	ring-cleaving dioxygenase	-
CDO84_RS21345	1205369..1205514	+	146	Protein_1155	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1165500..1201725	36225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29086.57 Da
• Isoelectric Point: 4.6145

>T77862 WP_040083357.1 NZ_CP021911:c1200614-1199868 [Bacillus sp. MD-5]
MVLFFQIMVWCIVAGLGLYVYATWRFESKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWDRYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CQYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKMVLIPFTIETKNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T77862 NZ_CP021911:c1200614-1199868 [Bacillus sp. MD-5]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATTGTGGCTGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
ATCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACATGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGATCGGTATCTCATTGTCGCAGTCAGCTTTGCTTTGATTGATGCTTTC
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCTGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGCATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCAATATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAATGGTTCTCATCCCTTTTACCATCGAGACAAAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTCGATTATTTGCTATTTACGTCATTAACAAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6555.59 Da
• Isoelectric Point: 10.8401

>AT77862 WP_003239095.1 NZ_CP021911:c1199868-1199698 [Bacillus sp. MD-5]
MERAFQNGCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT77862 NZ_CP021911:c1199868-1199698 [Bacillus sp. MD-5]
ATGGAACGTGCGTTTCAAAACGGATGCGAGCCCAGAGCGGCCAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCGAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: G4NV07