Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 349465..350101 Replicon chromosome
Accession NZ_CP021911
Organism Bacillus sp. MD-5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CDO84_RS01625 Protein ID WP_003156187.1
Coordinates 349751..350101 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag CDO84_RS01620 Protein ID WP_040084113.1
Coordinates 349465..349746 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO84_RS01600 345824..346423 - 600 WP_040084115.1 rhomboid family intramembrane serine protease -
CDO84_RS01605 346518..346883 + 366 WP_014475874.1 holo-ACP synthase -
CDO84_RS01610 347049..348065 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
CDO84_RS01615 348180..349349 + 1170 WP_040084114.1 alanine racemase -
CDO84_RS01620 349465..349746 + 282 WP_040084113.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CDO84_RS01625 349751..350101 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CDO84_RS01630 350216..351040 + 825 WP_014662853.1 RsbT co-antagonist protein RsbRA -
CDO84_RS01635 351045..351410 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
CDO84_RS01640 351414..351815 + 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
CDO84_RS01645 351827..352834 + 1008 WP_014662854.1 phosphoserine phosphatase RsbU -
CDO84_RS01650 352896..353225 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
CDO84_RS01655 353222..353704 + 483 WP_003234299.1 anti-sigma B factor RsbW -
CDO84_RS01660 353670..354458 + 789 WP_014662857.1 RNA polymerase sigma factor SigB -
CDO84_RS01665 354458..355057 + 600 WP_040084112.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T77861 WP_003156187.1 NZ_CP021911:349751-350101 [Bacillus sp. MD-5]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T77861 NZ_CP021911:349751-350101 [Bacillus sp. MD-5]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10516.90 Da        Isoelectric Point: 6.4708

>AT77861 WP_040084113.1 NZ_CP021911:349465-349746 [Bacillus sp. MD-5]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTARHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT77861 NZ_CP021911:349465-349746 [Bacillus sp. MD-5]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAGCTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure

References