Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2116553..2117082 Replicon chromosome
Accession NZ_CP021907
Organism Staphylococcus aureus strain Seattle 1945 isolate G478

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag CD795_RS10805 Protein ID WP_000621176.1
Coordinates 2116553..2116915 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag CD795_RS10810 Protein ID WP_000948331.1
Coordinates 2116912..2117082 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CD795_RS10765 2112218..2112601 - 384 WP_000358144.1 hypothetical protein -
CD795_RS10770 2112613..2112873 - 261 WP_000015640.1 hypothetical protein -
CD795_RS10775 2112878..2113945 - 1068 WP_000692013.1 conjugal transfer protein -
CD795_RS10780 2114006..2115097 - 1092 WP_000172936.1 replication initiation factor domain-containing protein -
CD795_RS10785 2115272..2115574 - 303 WP_000386891.1 hypothetical protein -
CD795_RS10790 2115588..2115908 - 321 WP_000805733.1 DUF961 family protein -
CD795_RS10795 2116059..2116343 - 285 WP_000134551.1 hypothetical protein -
CD795_RS10805 2116553..2116915 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CD795_RS10810 2116912..2117082 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
CD795_RS10815 2117167..2118315 - 1149 WP_001281140.1 alanine racemase -
CD795_RS10820 2118381..2118740 - 360 WP_000581197.1 holo-ACP synthase -
CD795_RS10825 2118744..2119235 - 492 WP_001286801.1 PH domain-containing protein -
CD795_RS10830 2119228..2120805 - 1578 WP_001294646.1 PH domain-containing protein -
CD795_RS10835 2120798..2121277 - 480 WP_001287082.1 hypothetical protein -
CD795_RS10840 2121486..2122046 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T77853 WP_000621176.1 NZ_CP021907:c2116915-2116553 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T77853 NZ_CP021907:c2116915-2116553 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT77853 WP_000948331.1 NZ_CP021907:c2117082-2116912 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT77853 NZ_CP021907:c2117082-2116912 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References