Detailed information of TA system TA077846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 364128..364663
• Replicon: chromosome
• Accession: NZ_CP021907
• Organism: Staphylococcus aureus strain Seattle 1945 isolate G478

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: A0A4P7P340
• Locus tag: CD795_RS01680
• Protein ID: WP_001103945.1
• Coordinates: 364340..364663 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: -
• Locus tag: CD795_RS01675
• Protein ID: WP_001058486.1
• Coordinates: 364128..364337 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD795_RS01640	359675..359917	+	243	WP_000897044.1	30S ribosomal protein S18	-
CD795_RS01645	360149..360982	-	834	WP_000370312.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CD795_RS01650	360983..362197	-	1215	WP_000270132.1	site-specific integrase	-
CD795_RS01655	362653..363348	-	696	WP_000668212.1	helix-turn-helix domain-containing protein	-
CD795_RS01660	363492..363704	+	213	WP_000794656.1	helix-turn-helix transcriptional regulator	-
CD795_RS01665	363705..363977	+	273	WP_000091734.1	helix-turn-helix domain-containing protein	-
CD795_RS01670	363989..364135	+	147	WP_031824147.1	hypothetical protein	-
CD795_RS01675	364128..364337	+	210	WP_001058486.1	hypothetical protein	Antitoxin
CD795_RS01680	364340..364663	+	324	WP_001103945.1	DUF1474 family protein	Toxin
CD795_RS01685	364728..365597	+	870	WP_001002723.1	primase alpha helix C-terminal domain-containing protein	-
CD795_RS01690	365611..367320	+	1710	WP_000447449.1	DUF927 domain-containing protein	-
CD795_RS01695	367632..368012	+	381	WP_000356936.1	hypothetical protein	-
CD795_RS01700	368009..368650	+	642	WP_001019780.1	hypothetical protein	-
CD795_RS01705	369167..369508	+	342	WP_001190612.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	355060..378532	23472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12665.26 Da
• Isoelectric Point: 4.7124

>T77846 WP_001103945.1 NZ_CP021907:364340-364663 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLQVYLKEFDELIQKF
HEIEKASSDVSLATESDDAKNSIKVAE

Nucleotide

• Length: 324 bp

>T77846 NZ_CP021907:364340-364663 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGA
GTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8252.31 Da
• Isoelectric Point: 4.3718

>AT77846 WP_001058486.1 NZ_CP021907:364128-364337 [Staphylococcus aureus]
MNNEQKEVIEHLVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT77846 NZ_CP021907:364128-364337 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGTGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7P340