Detailed information of TA system TA077821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3442172..3442768
• Replicon: chromosome
• Accession: NZ_CP021902
• Organism: Enterobacter cloacae complex sp. strain AR_0136

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: AM383_RS17840
• Protein ID: WP_023315213.1
• Coordinates: 3442466..3442768 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AM383_RS17835
• Protein ID: WP_032610152.1
• Coordinates: 3442172..3442459 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM383_RS17830 (AM383_17710)	3440544..3442175	+	1632	WP_032610151.1	Na/Pi cotransporter family protein	-
AM383_RS17835 (AM383_17715)	3442172..3442459	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
AM383_RS17840 (AM383_17720)	3442466..3442768	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM383_RS17845 (AM383_17725)	3442966..3443838	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
AM383_RS17850 (AM383_17730)	3443839..3444111	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
AM383_RS17855 (AM383_17735)	3444162..3445106	-	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
AM383_RS17860 (AM383_17740)	3445200..3446549	-	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T77821 WP_023315213.1 NZ_CP021902:c3442768-3442466 [Enterobacter cloacae complex sp.]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T77821 NZ_CP021902:c3442768-3442466 [Enterobacter cloacae complex sp.]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT77821 WP_032610152.1 NZ_CP021902:c3442459-3442172 [Enterobacter cloacae complex sp.]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT77821 NZ_CP021902:c3442459-3442172 [Enterobacter cloacae complex sp.]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8