Detailed information of TA system TA077820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2931877..2932484
• Replicon: chromosome
• Accession: NZ_CP021902
• Organism: Enterobacter cloacae complex sp. strain AR_0136

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4Q4AAS0
• Locus tag: AM383_RS15390
• Protein ID: WP_057979982.1
• Coordinates: 2932299..2932484 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM383_RS15385
• Protein ID: WP_023315016.1
• Coordinates: 2931877..2932284 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM383_RS15370 (AM383_15255)	2928620..2929873	+	1254	WP_015572749.1	valine--pyruvate transaminase	-
AM383_RS15375 (AM383_15260)	2929881..2930336	-	456	WP_023323869.1	4Fe-4S dicluster domain-containing protein	-
AM383_RS15380 (AM383_15265)	2930797..2931873	+	1077	WP_023323870.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
AM383_RS15385 (AM383_15270)	2931877..2932284	-	408	WP_023315016.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM383_RS15390 (AM383_15275)	2932299..2932484	-	186	WP_057979982.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM383_RS15395 (AM383_15280)	2932734..2934272	-	1539	WP_006808612.1	aldehyde dehydrogenase AldB	-
AM383_RS15400 (AM383_15285)	2934439..2935323	+	885	WP_003860164.1	ROK family protein	-
AM383_RS15405 (AM383_15290)	2935327..2937165	-	1839	WP_023323871.1	selenocysteine-specific translation elongation factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6852.08 Da
• Isoelectric Point: 11.5191

>T77820 WP_057979982.1 NZ_CP021902:c2932484-2932299 [Enterobacter cloacae complex sp.]
VKSADIITVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T77820 NZ_CP021902:c2932484-2932299 [Enterobacter cloacae complex sp.]
GTGAAAAGTGCGGATATCATTACTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTTACCGTCCCACATCCTAAAAAGGACATTAAACCAGGCACTCTCGCACAAATCT
GGCGACAGGCGGGAATTAAACACTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15205.20 Da
• Isoelectric Point: 4.5036

>AT77820 WP_023315016.1 NZ_CP021902:c2932284-2931877 [Enterobacter cloacae complex sp.]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEMLPLPGMVEAHLASHPEDFIGGQWL
LVDINMKQFEGRVERINITIPRRLLVKIDSFVSEHPQFTNRSAFLAEAARRVLPR

Nucleotide

• Length: 408 bp

>AT77820 NZ_CP021902:c2932284-2931877 [Enterobacter cloacae complex sp.]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGTGGTTTCTTTCCTGACGTTCCGGGGTGTTTTTT
TGCGGGGGATTCACTGGATGATGCTTTTCAGGATGCACGTGATGCATTAACTGCCCATTTTGAAGCGTTGTTTGAAATGG
ATGAAATGTTGCCACTTCCGGGTATGGTTGAAGCGCATCTGGCAAGTCATCCGGAGGATTTCATTGGCGGACAATGGTTG
CTGGTGGATATCAATATGAAACAGTTTGAAGGCAGAGTAGAGCGCATCAATATTACGATACCTCGCCGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCACAATTTACTAACCGAAGTGCTTTTCTTGCAGAGGCAGCACGGCGTGTACTGC
CCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A4Q4AAS0