Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 653424..654163 | Replicon | chromosome |
| Accession | NZ_CP021902 | ||
| Organism | Enterobacter cloacae complex sp. strain AR_0136 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A837FFP9 |
| Locus tag | AM383_RS04060 | Protein ID | WP_032609220.1 |
| Coordinates | 653424..653909 (-) | Length | 162 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | A0A837FCR9 |
| Locus tag | AM383_RS04065 | Protein ID | WP_003857131.1 |
| Coordinates | 653897..654163 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AM383_RS04035 (AM383_03965) | 648744..649559 | - | 816 | WP_012695470.1 | aminoglycoside O-phosphotransferase APH(3')-Ia | - |
| AM383_RS04040 (AM383_03970) | 649720..650688 | - | 969 | WP_013815099.1 | IS5-like element IS903B family transposase | - |
| AM383_RS04045 (AM383_03975) | 650720..651091 | - | 372 | Protein_650 | IS5/IS1182 family transposase | - |
| AM383_RS04050 (AM383_03980) | 651166..651798 | + | 633 | WP_001567369.1 | hypothetical protein | - |
| AM383_RS04055 (AM383_03985) | 651827..653230 | - | 1404 | WP_001567368.1 | ISNCY-like element ISKpn21 family transposase | - |
| AM383_RS04060 (AM383_03990) | 653424..653909 | - | 486 | WP_032609220.1 | GNAT family N-acetyltransferase | Toxin |
| AM383_RS04065 (AM383_03995) | 653897..654163 | - | 267 | WP_003857131.1 | DUF1778 domain-containing protein | Antitoxin |
| AM383_RS04070 (AM383_04000) | 654227..655156 | - | 930 | WP_032609221.1 | LysR family transcriptional regulator | - |
| AM383_RS04075 (AM383_04005) | 655286..656674 | + | 1389 | WP_032609222.1 | MFS transporter | - |
| AM383_RS04080 (AM383_04010) | 656696..657691 | - | 996 | WP_023314163.1 | DUF2891 domain-containing protein | - |
| AM383_RS04085 (AM383_04015) | 657701..658687 | - | 987 | WP_003857123.1 | DUF979 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | aph(3')-Ia / dfrA14 | - | 609839..780766 | 170927 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 162 a.a. Molecular weight: 17554.26 Da Isoelectric Point: 9.9658
>T77808 WP_032609220.1 NZ_CP021902:c653909-653424 [Enterobacter cloacae complex sp.]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVICKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVICKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q
Download Length: 486 bp
>T77808 NZ_CP021902:c653909-653424 [Enterobacter cloacae complex sp.]
GTGGGACGTGTAACAGCGCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCATTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCACTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCCTCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG
GTGGGACGTGTAACAGCGCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCATTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCACTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCCTCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG
Antitoxin
Download Length: 89 a.a. Molecular weight: 10090.50 Da Isoelectric Point: 4.3837
>AT77808 WP_003857131.1 NZ_CP021902:c654163-653897 [Enterobacter cloacae complex sp.]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV
Download Length: 267 bp
>AT77808 NZ_CP021902:c654163-653897 [Enterobacter cloacae complex sp.]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A837FFP9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A837FCR9 |