Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 38322..38965 Replicon plasmid tig00001437_pilon
Accession NZ_CP021900
Organism Enterobacter cloacae complex sp. strain AR_0136

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM383_RS00250 Protein ID WP_001044770.1
Coordinates 38549..38965 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM383_RS00245 Protein ID WP_001261282.1
Coordinates 38322..38552 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM383_RS00215 (AM383_00195) 34039..34794 - 756 WP_012539983.1 replication initiation protein RepE -
AM383_RS25445 35425..35538 + 114 WP_032072094.1 hypothetical protein -
AM383_RS00225 (AM383_00205) 35667..35924 + 258 WP_032408936.1 hypothetical protein -
AM383_RS00230 (AM383_00210) 35982..36767 - 786 WP_022644886.1 site-specific integrase -
AM383_RS00235 (AM383_00215) 36770..37408 - 639 WP_022644887.1 hypothetical protein -
AM383_RS25145 37457..37777 - 321 WP_032072060.1 hypothetical protein -
AM383_RS00240 (AM383_00220) 37905..38365 - 461 Protein_43 hypothetical protein -
AM383_RS00245 (AM383_00225) 38322..38552 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AM383_RS00250 (AM383_00230) 38549..38965 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM383_RS00255 (AM383_00235) 39039..39257 + 219 Protein_46 AAA family ATPase -
AM383_RS00260 (AM383_00240) 39322..40026 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
AM383_RS00270 (AM383_00250) 40078..41844 + 1767 Protein_48 Tn3 family transposase -
AM383_RS00275 (AM383_00255) 41923..42927 + 1005 WP_000427619.1 IS110-like element IS5075 family transposase -
AM383_RS25635 43199..43285 - 87 Protein_50 Arm DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaKPC-3 - 1..63482 63482


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T77807 WP_001044770.1 NZ_CP021900:38549-38965 [Enterobacter cloacae complex sp.]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T77807 NZ_CP021900:38549-38965 [Enterobacter cloacae complex sp.]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT77807 WP_001261282.1 NZ_CP021900:38322-38552 [Enterobacter cloacae complex sp.]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT77807 NZ_CP021900:38322-38552 [Enterobacter cloacae complex sp.]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References