Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1940799..1941420 Replicon chromosome
Accession NZ_CP021896
Organism Enterobacter cloacae complex sp. strain AR_0050

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM429_RS09405 Protein ID WP_015571250.1
Coordinates 1940799..1941017 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM429_RS09410 Protein ID WP_006809850.1
Coordinates 1941046..1941420 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM429_RS09375 (AM429_09340) 1936811..1937071 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AM429_RS09380 (AM429_09345) 1937074..1937214 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM429_RS09385 (AM429_09350) 1937211..1937921 - 711 WP_022647267.1 GNAT family protein -
AM429_RS09390 (AM429_09355) 1938024..1939484 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
AM429_RS09395 (AM429_09360) 1939456..1939923 - 468 WP_022647269.1 YlaC family protein -
AM429_RS09400 (AM429_09365) 1940040..1940591 - 552 WP_022647270.1 maltose O-acetyltransferase -
AM429_RS09405 (AM429_09370) 1940799..1941017 - 219 WP_015571250.1 HHA domain-containing protein Toxin
AM429_RS09410 (AM429_09375) 1941046..1941420 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM429_RS09415 (AM429_09380) 1941930..1945076 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
AM429_RS09420 (AM429_09385) 1945099..1946292 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T77796 WP_015571250.1 NZ_CP021896:c1941017-1940799 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T77796 NZ_CP021896:c1941017-1940799 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT77796 WP_006809850.1 NZ_CP021896:c1941420-1941046 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT77796 NZ_CP021896:c1941420-1941046 [Enterobacter cloacae complex sp.]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References