Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 7228..8009 Replicon chromosome
Accession NZ_CP021896
Organism Enterobacter cloacae complex sp. strain AR_0050

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A431SHU0
Locus tag AM429_RS00035 Protein ID WP_022649378.1
Coordinates 7228..7719 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A3S0FY21
Locus tag AM429_RS00040 Protein ID WP_022649379.1
Coordinates 7716..8009 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM429_RS00020 (AM429_00020) 2508..2960 - 453 WP_022649375.1 hypothetical protein -
AM429_RS00025 (AM429_00025) 3475..5385 + 1911 WP_022649376.1 hypothetical protein -
AM429_RS00030 (AM429_00030) 5382..7076 + 1695 WP_022649377.1 hypothetical protein -
AM429_RS00035 (AM429_00035) 7228..7719 - 492 WP_022649378.1 GNAT family N-acetyltransferase Toxin
AM429_RS00040 (AM429_00040) 7716..8009 - 294 WP_022649379.1 DUF1778 domain-containing protein Antitoxin
AM429_RS00045 (AM429_00045) 8111..9904 - 1794 WP_022649380.1 hybrid sensor histidine kinase/response regulator -
AM429_RS00050 (AM429_00050) 9901..10533 - 633 WP_022649381.1 response regulator transcription factor -
AM429_RS00055 (AM429_00055) 10709..11023 + 315 WP_003862457.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(83-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17469.36 Da        Isoelectric Point: 8.1405

>T77793 WP_022649378.1 NZ_CP021896:c7719-7228 [Enterobacter cloacae complex sp.]
MISAPVPLRAGHILTPFCCGVDSMDNWLKQRAMKNQASGASRTFVCCGGDSNVLAYYALASSAVTTNTVPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAESIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLV
ASI

Download         Length: 492 bp

>T77793 NZ_CP021896:c7719-7228 [Enterobacter cloacae complex sp.]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10959.58 Da        Isoelectric Point: 8.6351

>AT77793 WP_022649379.1 NZ_CP021896:c8009-7716 [Enterobacter cloacae complex sp.]
MPAANTMAVKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLTRLDQAPAPN
AALRKTMQTLAPWEQEK

Download         Length: 294 bp

>AT77793 NZ_CP021896:c8009-7716 [Enterobacter cloacae complex sp.]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SHU0

Antitoxin

Source ID Structure
AlphaFold DB A0A3S0FY21

References