Detailed information of TA system TA077791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 509423..510059
• Replicon: chromosome
• Accession: NZ_CP021892
• Organism: Bacillus subtilis subsp. subtilis strain SRCM100333

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: S100333_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 509709..510059 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: S100333_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 509423..509704 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S100333_RS02600	505782..506381	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
S100333_RS02605	506476..506841	+	366	WP_014478897.1	holo-ACP synthase	-
S100333_RS02610	507007..508023	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
S100333_RS02615	508138..509307	+	1170	WP_014478898.1	alanine racemase	-
S100333_RS02620	509423..509704	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
S100333_RS02625	509709..510059	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
S100333_RS02630	510164..510988	+	825	WP_088272172.1	RsbT co-antagonist protein RsbRA	-
S100333_RS02635	510993..511358	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
S100333_RS02640	511362..511763	+	402	WP_017697001.1	serine/threonine-protein kinase RsbT	-
S100333_RS02645	511775..512782	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
S100333_RS02650	512844..513173	+	330	WP_014662855.1	anti-sigma factor antagonist RsbV	-
S100333_RS02655	513170..513652	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
S100333_RS02660	513618..514406	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
S100333_RS02665	514406..515005	+	600	WP_017696999.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T77791 WP_003156187.1 NZ_CP021892:509709-510059 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T77791 NZ_CP021892:509709-510059 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT77791 WP_003225183.1 NZ_CP021892:509423-509704 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT77791 NZ_CP021892:509423-509704 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32