Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1601481..1602052 Replicon chromosome
Accession NZ_CP021885
Organism Enterococcus faecium strain WEFA23

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag CDW54_RS08110 Protein ID WP_002302307.1
Coordinates 1601711..1602052 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag CDW54_RS08105 Protein ID WP_002323011.1
Coordinates 1601481..1601711 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDW54_RS08075 1596970..1598298 + 1329 WP_010718318.1 FAD-dependent oxidoreductase -
CDW54_RS08080 1598320..1598946 + 627 WP_002286816.1 cysteine hydrolase -
CDW54_RS08085 1599129..1599710 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
CDW54_RS08090 1600075..1600650 + 576 WP_010718317.1 SOS response-associated peptidase family protein -
CDW54_RS08095 1600855..1601193 - 339 WP_002286804.1 hypothetical protein -
CDW54_RS08105 1601481..1601711 + 231 WP_002323011.1 hypothetical protein Antitoxin
CDW54_RS08110 1601711..1602052 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CDW54_RS08115 1602902..1603087 + 186 WP_002304207.1 hypothetical protein -
CDW54_RS12745 1604723..1604848 + 126 WP_002352139.1 LPXTG cell wall anchor domain-containing protein -
CDW54_RS08135 1604922..1605815 + 894 WP_002326335.1 class C sortase -
CDW54_RS08140 1605999..1606673 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL ebpC 1532747..1736568 203821


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T77788 WP_002302307.1 NZ_CP021885:1601711-1602052 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T77788 NZ_CP021885:1601711-1602052 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT77788 WP_002323011.1 NZ_CP021885:1601481-1601711 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT77788 NZ_CP021885:1601481-1601711 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References