Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 33025..33550 Replicon plasmid tig00001069_pilon
Accession NZ_CP021880
Organism Escherichia coli strain AR_0137

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AM384_RS24545 Protein ID WP_001159871.1
Coordinates 33245..33550 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag AM384_RS24540 Protein ID WP_000813630.1
Coordinates 33025..33243 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM384_RS24500 28235..28921 + 687 Protein_28 IS1 family transposase -
AM384_RS24510 29175..30197 - 1023 WP_000361402.1 helicase UvrD -
AM384_RS24515 30182..31747 - 1566 WP_001128474.1 AAA family ATPase -
AM384_RS24520 31822..32238 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
AM384_RS24525 32235..32465 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AM384_RS24540 33025..33243 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM384_RS24545 33245..33550 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AM384_RS24550 33551..34357 + 807 WP_000016970.1 site-specific integrase -
AM384_RS24555 35079..35834 + 756 WP_000852146.1 replication initiation protein RepE -
AM384_RS24560 36422..37588 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / aadA5 / qacE / sul1 / mph(A) / tet(B) iucA / iucB / iucC / iucD / iutA 1..169449 169449
- flank IS/Tn - - 28574..28921 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T77774 WP_001159871.1 NZ_CP021880:33245-33550 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77774 NZ_CP021880:33245-33550 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT77774 WP_000813630.1 NZ_CP021880:33025-33243 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77774 NZ_CP021880:33025-33243 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References