Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2023031..2023865 Replicon chromosome
Accession NZ_CP021879
Organism Escherichia coli strain AR_0137

Toxin (Protein)


Gene name yeeV Uniprot ID W0S379
Locus tag AM384_RS10380 Protein ID WP_000854808.1
Coordinates 2023488..2023865 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2J4HRK6
Locus tag AM384_RS10375 Protein ID WP_001354276.1
Coordinates 2023031..2023399 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM384_RS10350 2020692..2021510 + 819 WP_001234702.1 DUF945 domain-containing protein -
AM384_RS10355 2021601..2022086 + 486 WP_000214319.1 antirestriction protein -
AM384_RS10360 2022102..2022578 + 477 WP_001354275.1 RadC family protein -
AM384_RS10365 2022647..2022868 + 222 WP_000692345.1 DUF987 domain-containing protein -
AM384_RS10375 2023031..2023399 + 369 WP_001354276.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
AM384_RS10380 2023488..2023865 + 378 WP_000854808.1 type IV toxin-antitoxin system toxin CbtA Toxin
AM384_RS10385 2023862..2024350 + 489 WP_000761645.1 hypothetical protein -
AM384_RS10390 2024362..2024559 + 198 WP_000839241.1 DUF957 domain-containing protein -
AM384_RS10395 2024644..2025489 + 846 WP_001280440.1 DUF4942 domain-containing protein -
AM384_RS10405 2025788..2026294 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
AM384_RS10410 2026381..2027727 + 1347 WP_000483767.1 IS4-like element IS4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2026399..2027727 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13909.93 Da        Isoelectric Point: 7.9085

>T77756 WP_000854808.1 NZ_CP021879:2023488-2023865 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T77756 NZ_CP021879:2023488-2023865 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGTCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13649.47 Da        Isoelectric Point: 7.0261

>AT77756 WP_001354276.1 NZ_CP021879:2023031-2023399 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Download         Length: 369 bp

>AT77756 NZ_CP021879:2023031-2023399 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCAAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W0S379

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4HRK6

References