Detailed information of TA system TA077748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2438185..2439216
• Replicon: chromosome
• Accession: NZ_CP021874
• Organism: Enterococcus wangshanyuanii strain MN05

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: CC204_RS12060
• Protein ID: WP_088270391.1
• Coordinates: 2438566..2439216 (+)
• Length: 217 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: CC204_RS12055
• Protein ID: WP_088270390.1
• Coordinates: 2438185..2438553 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CC204_RS12045	2435636..2436634	-	999	WP_088270388.1	hypothetical protein	-
CC204_RS21395	2436696..2436863	-	168	WP_188634510.1	hypothetical protein	-
CC204_RS12055	2438185..2438553	+	369	WP_088270390.1	helix-turn-helix transcriptional regulator	Antitoxin
CC204_RS12060	2438566..2439216	+	651	WP_088270391.1	ImmA/IrrE family metallo-endopeptidase	Toxin
CC204_RS12065	2439352..2441001	+	1650	WP_088270392.1	hypothetical protein	-
CC204_RS12070	2441385..2441681	-	297	WP_154776777.1	DUF4176 domain-containing protein	-
CC204_RS12075	2441681..2442337	-	657	WP_157894276.1	DUF443 family protein	-
CC204_RS12080	2442344..2443744	-	1401	WP_088270395.1	hypothetical protein	-
CC204_RS12085	2443744..2444088	-	345	WP_088270396.1	DUF3958 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2277654..2456817	179163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (74-175)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-61)

Sequences

Toxin

Protein

• Length: 217 a.a.
• Molecular weight: 25604.42 Da
• Isoelectric Point: 6.3409

>T77748 WP_088270391.1 NZ_CP021874:2438566-2439216 [Enterococcus wangshanyuanii]
MDFINEELYFEAFNLANELTKEVAYYCSKKIEEVKCFDIEKYVKELEDVEFIDYPFQKQLKNKMLGSTSKVYGEIIITTN
KNLMIERKNFTKMHEIVHFYRDIPYVSESHTFSDMIIKNGYFPEDIPREYRANVGASILLANDRALLFALKQFNTFQQVA
DFFFMSMSALQNRLKEHLVFVCNCTPKYAFHLVYNYRIGNGKELFSIVYSQVNYQS

Nucleotide

• Length: 651 bp

>T77748 NZ_CP021874:2438566-2439216 [Enterococcus wangshanyuanii]
TTGGATTTCATTAACGAGGAACTATATTTTGAAGCCTTCAATCTTGCCAACGAATTAACAAAAGAAGTCGCTTATTATTG
TTCAAAAAAGATTGAAGAAGTAAAATGTTTTGACATTGAAAAGTATGTAAAAGAACTAGAAGACGTTGAATTTATTGATT
ACCCTTTTCAAAAACAATTAAAAAATAAAATGCTAGGCTCCACTTCTAAAGTTTATGGAGAAATCATTATTACAACTAAT
AAGAATTTAATGATTGAAAGAAAAAATTTCACGAAAATGCACGAAATAGTTCATTTCTACAGAGATATACCTTATGTTAG
TGAATCACACACATTTTCTGACATGATAATTAAAAATGGTTATTTTCCAGAAGATATACCTAGAGAATATCGAGCTAATG
TAGGTGCAAGCATCCTTCTAGCAAATGATCGCGCTTTATTATTTGCTTTGAAGCAATTCAACACCTTTCAGCAAGTTGCA
GACTTCTTTTTCATGAGTATGTCAGCACTTCAGAACAGACTAAAAGAACATCTAGTATTTGTTTGTAATTGTACACCTAA
ATATGCCTTTCACTTAGTCTATAATTACCGTATTGGAAATGGTAAAGAACTTTTTAGCATAGTATATTCACAAGTAAATT
ATCAAAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14202.09 Da
• Isoelectric Point: 4.7452

>AT77748 WP_088270390.1 NZ_CP021874:2438185-2438553 [Enterococcus wangshanyuanii]
METYKLIRQLREEKELTQQEVANALKLNLSVYKKIELGSRPIRETELSNIADFFNVTTDFLLGRTSPITDSKIGDSITSH
FRLNTANMDIDDIEELEEELTDYMEFLIKKAKEKKAKRENNS

Nucleotide

• Length: 369 bp

>AT77748 NZ_CP021874:2438185-2438553 [Enterococcus wangshanyuanii]
ATGGAAACATACAAATTAATCAGGCAGTTAAGAGAAGAAAAAGAGTTAACCCAACAAGAAGTTGCTAATGCTCTTAAACT
CAATTTGAGTGTTTACAAAAAGATTGAACTCGGCTCTCGTCCTATAAGAGAAACTGAATTATCTAACATTGCTGATTTTT
TCAATGTGACAACTGATTTTCTTCTTGGAAGAACCTCCCCAATTACCGATAGTAAAATAGGAGATAGTATTACTTCTCAC
TTTAGATTAAACACTGCAAACATGGATATTGATGATATCGAGGAACTCGAAGAAGAATTAACTGATTACATGGAATTTTT
AATAAAGAAAGCAAAAGAAAAGAAAGCAAAAAGAGAAAATAATAGTTAG