Detailed information of TA system TA077747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 2144014..2145069
• Replicon: chromosome
• Accession: NZ_CP021874
• Organism: Enterococcus wangshanyuanii strain MN05

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: CC204_RS10630
• Protein ID: WP_088270106.1
• Coordinates: 2144413..2145069 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: CC204_RS10625
• Protein ID: WP_088270105.1
• Coordinates: 2144014..2144400 (+)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CC204_RS10615	2139227..2141665	-	2439	WP_088270103.1	LPXTG cell wall anchor domain-containing protein	-
CC204_RS10620	2141812..2142840	-	1029	WP_088270104.1	hypothetical protein	-
CC204_RS21390	2142902..2143069	-	168	WP_188634494.1	hypothetical protein	-
CC204_RS10625	2144014..2144400	+	387	WP_088270105.1	helix-turn-helix transcriptional regulator	Antitoxin
CC204_RS10630	2144413..2145069	+	657	WP_088270106.1	ImmA/IrrE family metallo-endopeptidase	Toxin
CC204_RS10635	2145124..2146197	-	1074	WP_088270107.1	hypothetical protein	-
CC204_RS10640	2146718..2147932	-	1215	WP_088270108.1	ATP-binding protein	-
CC204_RS10645	2147991..2149211	-	1221	WP_088270109.1	hypothetical protein	-
CC204_RS10650	2149295..2149525	-	231	WP_088271696.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2080926..2211256	130330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (73-175)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-62)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25802.53 Da
• Isoelectric Point: 5.7168

>T77747 WP_088270106.1 NZ_CP021874:2144413-2145069 [Enterococcus wangshanyuanii]
MDFINEELYFEAFNLANELTKDVAYYCSKKIEEVKCFDIEKYVKELEDVEFIDYPFQKQLKNKMLGSTSKVYGEVIITTN
RNLMIERKNFTKMHEIVHFYKDIPCVSESHTFSDMVIENGYFPEDIPREYRANVGASILLANNHALLSALRQFNTFQQVA
DFFFMSKSALQNRLKEYLIFICNCTPQYAFQLVYNYRIGNGKELFQVMYSSKNYYSLN

Nucleotide

• Length: 657 bp

>T77747 NZ_CP021874:2144413-2145069 [Enterococcus wangshanyuanii]
TTGGATTTCATTAACGAGGAACTATATTTTGAAGCCTTCAATCTTGCTAACGAGTTAACAAAAGATGTCGCTTATTATTG
TTCAAAAAAGATTGAAGAAGTCAAATGCTTTGACATTGAAAAATATGTAAAAGAACTAGAAGACGTTGAATTTATTGATT
ACCCTTTTCAAAAACAATTAAAAAATAAAATGCTAGGCTCCACTTCTAAAGTTTATGGAGAAGTCATTATTACAACTAAT
AGGAATTTAATGATTGAAAGAAAAAATTTCACCAAAATGCACGAAATAGTTCATTTCTACAAAGATATACCTTGTGTTAG
TGAATCACACACATTTTCTGACATGGTAATTGAAAATGGCTATTTCCCAGAGGATATACCTAGAGAATATCGAGCAAATG
TAGGTGCAAGTATTCTTCTAGCAAATAATCATGCATTACTATCTGCATTAAGACAATTCAATACATTTCAACAAGTTGCA
GATTTCTTTTTCATGAGTAAATCAGCACTACAAAATAGATTAAAAGAATATCTTATATTTATTTGTAATTGTACGCCTCA
ATATGCTTTTCAATTGGTCTATAATTACCGTATCGGAAATGGTAAAGAACTTTTCCAAGTGATGTATTCATCAAAAAATT
ATTACAGTTTGAATTAG

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14823.73 Da
• Isoelectric Point: 5.0516

>AT77747 WP_088270105.1 NZ_CP021874:2144014-2144400 [Enterococcus wangshanyuanii]
MIMFSERLLKMRKNAELTQEELAKKINVSKQSVGSWERGRTEPSIETITELANVLNTTTDYLLGRTNQPIQNNIDSTNIG
NNITSHFRLNTSDMNIDDIEELEEELTDYMEFLIKKAKEKKAKREKNS

Nucleotide

• Length: 387 bp

>AT77747 NZ_CP021874:2144014-2144400 [Enterococcus wangshanyuanii]
ATGATTATGTTTAGTGAAAGACTATTAAAAATGCGAAAAAATGCTGAATTAACACAAGAAGAACTAGCAAAAAAAATAAA
TGTTTCTAAACAAAGTGTCGGTAGTTGGGAACGTGGTAGAACAGAGCCATCAATAGAAACCATAACCGAATTAGCAAACG
TATTAAACACTACTACTGATTACTTACTAGGAAGAACTAACCAACCTATCCAGAATAATATTGATTCAACTAATATCGGC
AATAATATTACTTCACATTTTAGACTTAACACATCCGATATGAACATTGATGATATAGAAGAATTAGAAGAAGAATTAAC
TGATTACATGGAATTTTTAATAAAGAAAGCAAAAGAAAAGAAAGCAAAAAGAGAAAAGAATAGTTAG