Detailed information of TA system TA077735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 1242656..1243902
• Replicon: chromosome
• Accession: NZ_CP021870
• Organism: Streptococcus agalactiae strain SG-M4

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: -
• Locus tag: BB165_RS06500
• Protein ID: WP_000170757.1
• Coordinates: 1242656..1243426 (-)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: Q8E4Q7
• Locus tag: BB165_RS06505
• Protein ID: WP_000578715.1
• Coordinates: 1243426..1243902 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB165_RS06480	1237687..1238046	-	360	WP_011074740.1	hypothetical protein	-
BB165_RS06490	1238381..1240399	-	2019	WP_000222644.1	ATP-dependent helicase	-
BB165_RS06495	1240403..1242643	-	2241	WP_000281027.1	AAA family ATPase	-
BB165_RS06500	1242656..1243426	-	771	WP_000170757.1	zeta toxin family protein	Toxin
BB165_RS06505	1243426..1243902	-	477	WP_000578715.1	helix-turn-helix domain-containing protein	Antitoxin
BB165_RS06510	1243972..1244262	-	291	WP_001210630.1	hypothetical protein	-
BB165_RS06515	1244309..1244698	-	390	WP_088203060.1	chemotaxis protein	-
BB165_RS06520	1244695..1244925	-	231	WP_000110918.1	hypothetical protein	-
BB165_RS06525	1244980..1246065	-	1086	WP_000161120.1	toprim domain-containing protein	-
BB165_RS06530	1246106..1246744	-	639	WP_000932868.1	hypothetical protein	-
BB165_RS06535	1246909..1247208	-	300	WP_001147539.1	hypothetical protein	-
BB165_RS06540	1247219..1247518	-	300	WP_000084342.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	wbtL / lmb / scpB	1163818..1257576	93758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29425.53 Da
• Isoelectric Point: 8.3930

>T77735 WP_000170757.1 NZ_CP021870:c1243426-1242656 [Streptococcus agalactiae]
MTLAEYSEAEFQKALRRNIRALTRGKTISSTPKAVLLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSFRSQHPNYLALQE
EYGKYSVDYTKDFAGKMVEHLIGELSKQSYHLLIEGTLRTTEVPRKTAQLLKSRGYQVSLALIATKPELSYLSTLIRYEE
LYAIDPNQARATPKEYHDGIVEHLVDNLRELENDKLFNRIQIYQRDRHCVYDSETDKGIAAEVLQECLFGKWSKVEEEML
RVGQERLRELSMRTPK

Nucleotide

• Length: 771 bp

>T77735 NZ_CP021870:c1243426-1242656 [Streptococcus agalactiae]
ATGACATTAGCAGAATATAGTGAGGCGGAGTTTCAAAAGGCTTTAAGGCGTAATATTCGTGCTTTAACCCGTGGCAAAAC
TATTTCCTCAACTCCCAAAGCAGTTCTGCTTGGTGGTCAAAGTGGAGCTGGAAAAACGACCATTCATCGAATCAAGCAAA
AGGAATTTCAAGGGAATATCATCATTATTGACGGAGATAGCTTCCGTTCGCAGCATCCAAATTATCTGGCTTTGCAGGAA
GAGTATGGCAAATATAGCGTTGATTATACCAAAGATTTTGCGGGGAAGATGGTTGAACACCTCATTGGTGAACTCAGCAA
ACAAAGCTATCATTTATTAATTGAGGGAACACTGAGAACAACAGAAGTCCCTCGAAAAACAGCTCAATTATTAAAATCAA
GAGGCTATCAAGTATCACTTGCGCTCATCGCAACAAAGCCAGAACTGTCCTATCTTAGCACCTTGATACGCTATGAAGAA
CTTTATGCCATAGATCCTAATCAAGCCAGAGCAACACCAAAAGAATATCATGATGGGATTGTGGAACACTTGGTTGATAA
TCTACGAGAGTTAGAAAATGATAAACTTTTTAACCGGATTCAAATCTATCAAAGAGATAGACATTGTGTCTATGATTCTG
AAACTGATAAAGGCATAGCTGCAGAAGTGCTTCAAGAATGTCTCTTTGGGAAATGGAGTAAGGTAGAGGAGGAGATGTTG
AGGGTGGGACAGGAACGGTTGAGGGAGTTAAGTATGAGAACCCCAAAATAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18145.47 Da
• Isoelectric Point: 4.4655

>AT77735 WP_000578715.1 NZ_CP021870:c1243902-1243426 [Streptococcus agalactiae]
MIGDNIKSLRRTHDLTQPEFAKIIGISRNSLSRYENGTSSVSTELIDRICQKFNVSYVDIVGEDKMMTPVEDYQLTIKIE
VLKERGSAILSRLYRHQDEQGIAFDDESNPWILISDDVADLVNTKIYLVDTFEELERYNGYLDGIERMLNLANHQVVA

Nucleotide

• Length: 477 bp

>AT77735 NZ_CP021870:c1243902-1243426 [Streptococcus agalactiae]
ATGATTGGAGATAATATTAAGTCCTTACGACGAACACATGATTTAACGCAGCCAGAGTTCGCAAAAATTATTGGTATTTC
AAGGAATAGTCTTAGTCGTTATGAAAATGGAACCAGCTCCGTTTCAACCGAACTCATTGATCGGATTTGTCAGAAATTTA
ATGTGTCTTATGTCGATATTGTTGGAGAAGATAAGATGATGACACCCGTAGAAGATTATCAGCTGACTATAAAAATTGAA
GTACTGAAAGAACGAGGTTCTGCCATTTTGTCAAGGCTGTACCGCCATCAAGATGAGCAAGGGATTGCATTTGACGATGA
ATCGAATCCGTGGATTTTGATAAGTGATGATGTAGCAGATTTAGTGAATACCAAGATTTATCTTGTCGATACCTTTGAAG
AGTTAGAGCGTTACAATGGCTATTTAGATGGTATTGAGCGGATGTTAAACCTTGCCAATCACCAGGTGGTGGCCTAA

Structures

Antitoxin

• AlphaFold DB: Q8E4Q7