Detailed information of TA system TA077730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 706603..707846
• Replicon: chromosome
• Accession: NZ_CP021867
• Organism: Streptococcus agalactiae strain SG-M25

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: BB164_RS04155
• Protein ID: WP_001235242.1
• Coordinates: 707079..707846 (+)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: BB164_RS04150
• Protein ID: WP_000578719.1
• Coordinates: 706603..707079 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB164_RS04105	701730..702051	+	322	Protein_710	DUF4368 domain-containing protein	-
BB164_RS04110	702044..703003	+	960	Protein_711	hypothetical protein	-
BB164_RS04115	703074..703373	+	300	WP_050197324.1	hypothetical protein	-
BB164_RS04120	703387..703677	+	291	WP_050197323.1	hypothetical protein	-
BB164_RS04125	703773..704411	+	639	WP_050197322.1	hypothetical protein	-
BB164_RS04130	704451..705551	+	1101	WP_050197321.1	toprim domain-containing protein	-
BB164_RS04135	705579..705806	+	228	WP_000110922.1	hypothetical protein	-
BB164_RS04140	705803..706192	+	390	WP_000048066.1	hypothetical protein	-
BB164_RS04145	706243..706533	+	291	WP_000667951.1	hypothetical protein	-
BB164_RS04150	706603..707079	+	477	WP_000578719.1	helix-turn-helix domain-containing protein	Antitoxin
BB164_RS04155	707079..707846	+	768	WP_001235242.1	zeta toxin family protein	Toxin
BB164_RS04160	707883..710390	+	2508	WP_001213955.1	AAA family ATPase	-
BB164_RS04170	710728..711090	+	363	WP_000140674.1	hypothetical protein	-
BB164_RS04175	711093..711458	+	366	WP_000431640.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / erm(B)	gbs0628 / gbs0630	636436..793170	156734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T77730 WP_001235242.1 NZ_CP021867:707079-707846 [Streptococcus agalactiae]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T77730 NZ_CP021867:707079-707846 [Streptococcus agalactiae]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT77730 WP_000578719.1 NZ_CP021867:706603-707079 [Streptococcus agalactiae]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT77730 NZ_CP021867:706603-707079 [Streptococcus agalactiae]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: G7SEB4