Detailed information of TA system TA077721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 699699..700131
• Replicon: chromosome
• Accession: NZ_CP021862
• Organism: Streptococcus agalactiae strain CUGBS591

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: BB161_RS03920
• Protein ID: WP_001284310.1
• Coordinates: 699973..700131 (+)
• Length: 53 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: BB161_RS03915
• Protein ID: WP_000301765.1
• Coordinates: 699699..699971 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB161_RS03870	695313..695564	+	252	Protein_670	GNAT family N-acetyltransferase	-
BB161_RS03875	695657..696451	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
BB161_RS03880	696861..696944	+	84	Protein_672	peptide-binding protein	-
BB161_RS03885	696993..697076	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
BB161_RS03890	697201..697938	+	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
BB161_RS03900	698084..698368	+	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
BB161_RS03905	698478..699374	+	897	WP_001809760.1	ParA family protein	-
BB161_RS03910	699472..699681	+	210	WP_000527318.1	peptide-binding protein	-
BB161_RS03915	699699..699971	+	273	WP_000301765.1	antitoxin	Antitoxin
BB161_RS03920	699973..700131	+	159	WP_001284310.1	zeta toxin family protein	Toxin
BB161_RS03925	700183..700728	+	546	WP_001071501.1	AAA family ATPase	-
BB161_RS03930	700904..701770	+	867	WP_001255868.1	aminoglycoside 6-adenylyltransferase AadE	-
BB161_RS03945	702341..703009	+	669	Protein_682	zeta toxin family protein	-
BB161_RS03950	703160..703651	+	492	WP_000011313.1	hypothetical protein	-
BB161_RS03955	703644..704809	+	1166	Protein_684	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	cylX / cylD / cylG / acpC / cylZ / cylA / cylB / cylE / cylF / cylI / cylJ / cylK	619532..745848	126316
	-	inside	IScluster/Tn	ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	-	682140..701770	19630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-50)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5790.55 Da
• Isoelectric Point: 10.3088

>T77721 WP_001284310.1 NZ_CP021862:699973-700131 [Streptococcus agalactiae]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRRR

Nucleotide

• Length: 159 bp

>T77721 NZ_CP021862:699973-700131 [Streptococcus agalactiae]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGAAGAAGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT77721 WP_000301765.1 NZ_CP021862:699699-699971 [Streptococcus agalactiae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT77721 NZ_CP021862:699699-699971 [Streptococcus agalactiae]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3