Detailed information of TA system TA077720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 690951..692059
• Replicon: chromosome
• Accession: NZ_CP021862
• Organism: Streptococcus agalactiae strain CUGBS591

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: BB161_RS03845
• Protein ID: WP_071661597.1
• Coordinates: 691190..692059 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: BB161_RS03840
• Protein ID: WP_000205227.1
• Coordinates: 690951..691175 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB161_RS03815	686582..688066	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
BB161_RS03820	688120..688923	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
BB161_RS03830	689232..690533	-	1302	Protein_662	ISL3 family transposase	-
BB161_RS12000	690722..690808	+	87	Protein_663	site-specific recombinase	-
BB161_RS03840	690951..691175	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
BB161_RS03845	691190..692059	+	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
BB161_RS03850	692040..692774	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
BB161_RS03855	692807..693715	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
BB161_RS03860	693712..693918	+	207	WP_044116541.1	hypothetical protein	-
BB161_RS03865	693992..695154	+	1163	WP_088186165.1	IS3-like element ISSag12 family transposase	-
BB161_RS03870	695313..695564	+	252	Protein_670	GNAT family N-acetyltransferase	-
BB161_RS03875	695657..696451	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
BB161_RS03880	696861..696944	+	84	Protein_672	peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	cylX / cylD / cylG / acpC / cylZ / cylA / cylB / cylE / cylF / cylI / cylJ / cylK	619532..745848	126316
	-	inside	IScluster/Tn	ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	-	682140..701770	19630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T77720 WP_071661597.1 NZ_CP021862:691190-692059 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T77720 NZ_CP021862:691190-692059 [Streptococcus agalactiae]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGTATCAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAACATAAACCTGAAAAATATACCGAG
AAGGTAAATCATATTTTTGAAGTACTCGGTATCTCACTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT77720 WP_000205227.1 NZ_CP021862:690951-691175 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT77720 NZ_CP021862:690951-691175 [Streptococcus agalactiae]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6