Detailed information of TA system TA077719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 680284..681392
• Replicon: chromosome
• Accession: NZ_CP021862
• Organism: Streptococcus agalactiae strain CUGBS591

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: BB161_RS03775
• Protein ID: WP_002378351.1
• Coordinates: 680523..681392 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: BB161_RS03770
• Protein ID: WP_000205227.1
• Coordinates: 680284..680508 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB161_RS03740	675497..675670	+	174	WP_002779755.1	cysteine-rich KTR domain-containing protein	-
BB161_RS11915	675873..676043	+	171	WP_000713595.1	hypothetical protein	-
BB161_RS03745	676057..676659	+	603	WP_001062586.1	recombinase family protein	-
BB161_RS03750	676675..677352	+	678	Protein_646	DNA topoisomerase	-
BB161_RS11995	677663..677827	+	165	Protein_647	DNA recombinase	-
BB161_RS03755	677864..678160	+	297	WP_002360685.1	hypothetical protein	-
BB161_RS03760	678161..678577	+	417	WP_000323438.1	recombinase	-
BB161_RS03765	678597..680141	+	1545	WP_002390960.1	recombinase family protein	-
BB161_RS03770	680284..680508	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
BB161_RS03775	680523..681392	+	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
BB161_RS03780	681373..682107	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
BB161_RS03785	682140..683003	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
BB161_RS03790	683047..683574	+	528	WP_002294507.1	adenine phosphoribosyltransferase	-
BB161_RS03795	683706..684515	+	810	WP_002294509.1	ANT(9) family aminoglycoside nucleotidyltransferase Spw	-
BB161_RS03800	684733..684984	+	252	WP_002294510.1	hypothetical protein	-
BB161_RS03805	685035..685493	-	459	WP_002325145.1	hypothetical protein	-
BB161_RS03810	685579..686139	+	561	Protein_659	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	cylX / cylD / cylG / acpC / cylZ / cylA / cylB / cylE / cylF / cylI / cylJ / cylK	619532..745848	126316
	-	inside	IScluster/Tn	ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	-	682140..701770	19630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T77719 WP_002378351.1 NZ_CP021862:680523-681392 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T77719 NZ_CP021862:680523-681392 [Streptococcus agalactiae]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAGCTTTATAATGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT77719 WP_000205227.1 NZ_CP021862:680284-680508 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT77719 NZ_CP021862:680284-680508 [Streptococcus agalactiae]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A193AUR0