Detailed information of TA system TA077716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 37358..37851
• Replicon: plasmid tig00000009_pilon
• Accession: NZ_CP021861
• Organism: Klebsiella pneumoniae strain AR_0125

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: AM372_RS29030
• Protein ID: WP_001230709.1
• Coordinates: 37597..37851 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: AM372_RS29025
• Protein ID: WP_000151456.1
• Coordinates: 37358..37600 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM372_RS28960	32788..33225	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
AM372_RS28965	33250..33432	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AM372_RS28970	33977..34159	+	183	WP_001401700.1	hypothetical protein	-
AM372_RS28975	34546..34806	-	261	WP_020314977.1	hypothetical protein	-
AM372_RS28990	35341..35580	-	240	WP_022540736.1	transcriptional regulator	-
AM372_RS28995	35570..35830	-	261	WP_020314676.1	hypothetical protein	-
AM372_RS29000	35882..36106	-	225	WP_000814381.1	hypothetical protein	-
AM372_RS29005	36203..36412	-	210	WP_020314678.1	hypothetical protein	-
AM372_RS29010	36454..36777	-	324	WP_020314677.1	hypothetical protein	-
AM372_RS29025	37358..37600	-	243	WP_000151456.1	hypothetical protein	Antitoxin
AM372_RS29030	37597..37851	-	255	WP_001230709.1	hypothetical protein	Toxin
AM372_RS29035	37848..38390	-	543	WP_001083904.1	hypothetical protein	-
AM372_RS29040	38494..38820	-	327	Protein_47	J domain-containing protein	-
AM372_RS29045	38937..39125	+	189	WP_000957857.1	hypothetical protein	-
AM372_RS29050	39135..40334	+	1200	WP_000948429.1	IS91 family transposase	-
AM372_RS29055	40449..40697	-	249	WP_088207303.1	DnaJ domain-containing protein	-
AM372_RS29060	41101..41430	-	330	WP_001542022.1	hypothetical protein	-
AM372_RS29070	42017..42682	+	666	WP_000532148.1	P-loop NTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaKPC-3	-	1..79489	79489
	-	flank	IS/Tn	-	-	39135..40334	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T77716 WP_001230709.1 NZ_CP021861:c37851-37597 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T77716 NZ_CP021861:c37851-37597 [Klebsiella pneumoniae]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT77716 WP_000151456.1 NZ_CP021861:c37600-37358 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT77716 NZ_CP021861:c37600-37358 [Klebsiella pneumoniae]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5