Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36277..36802 Replicon plasmid tig00000002_pilon
Accession NZ_CP021857
Organism Klebsiella pneumoniae strain AR_0125

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag AM372_RS01010 Protein ID WP_001568026.1
Coordinates 36277..36582 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AM372_RS01015 Protein ID WP_001568025.1
Coordinates 36584..36802 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM372_RS00980 31985..32611 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
AM372_RS00985 32731..32910 + 180 WP_006788217.1 hypothetical protein -
AM372_RS00990 33358..34152 - 795 WP_004197635.1 site-specific integrase -
AM372_RS00995 34350..35366 - 1017 WP_020315256.1 hypothetical protein -
AM372_RS01000 35363..35686 - 324 WP_022644730.1 hypothetical protein -
AM372_RS01005 35713..36108 - 396 WP_017899885.1 hypothetical protein -
AM372_RS01010 36277..36582 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
AM372_RS01015 36584..36802 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM372_RS01025 37355..38044 + 690 WP_004193995.1 hypothetical protein -
AM372_RS01030 38076..38765 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
AM372_RS01035 39202..39471 + 270 WP_020804424.1 hypothetical protein -
AM372_RS01040 39459..40034 + 576 WP_020317495.1 hypothetical protein -
AM372_RS01045 40065..40559 + 495 WP_020802777.1 hypothetical protein -
AM372_RS01050 40603..40971 + 369 WP_004197807.1 hypothetical protein -
AM372_RS01055 41005..41208 + 204 WP_004197808.1 hemolysin expression modulator Hha -
AM372_RS01060 41285..41464 + 180 WP_050893391.1 hypothetical protein -
AM372_RS01065 41579..41758 - 180 Protein_47 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-12 - 1..106331 106331


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T77695 WP_001568026.1 NZ_CP021857:c36582-36277 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T77695 NZ_CP021857:c36582-36277 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT77695 WP_001568025.1 NZ_CP021857:c36802-36584 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT77695 NZ_CP021857:c36802-36584 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References