Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 37637..38373 Replicon plasmid tig00000001_pilon
Accession NZ_CP021856
Organism Klebsiella pneumoniae strain AR_0125

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM372_RS00240 Protein ID WP_003026803.1
Coordinates 37637..38119 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM372_RS00245 Protein ID WP_003026799.1
Coordinates 38107..38373 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM372_RS00225 33274..34236 + 963 WP_004152113.1 M48 family metalloprotease -
AM372_RS00230 34324..35578 + 1255 Protein_40 IS3 family transposase -
AM372_RS00235 36079..37425 - 1347 WP_020314316.1 ISNCY family transposase -
AM372_RS00240 37637..38119 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM372_RS00245 38107..38373 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM372_RS00250 38549..38803 - 255 WP_004152108.1 hypothetical protein -
AM372_RS00255 38879..39136 - 258 WP_004152107.1 hypothetical protein -
AM372_RS00260 39185..39388 - 204 WP_004152106.1 hemolysin expression modulator Hha -
AM372_RS00265 39422..39790 - 369 WP_004152105.1 hypothetical protein -
AM372_RS00270 39834..40328 - 495 WP_004152104.1 DNA-binding protein -
AM372_RS00275 40359..40934 - 576 WP_004152103.1 hypothetical protein -
AM372_RS00280 40922..41191 - 270 WP_004152102.1 hypothetical protein -
AM372_RS00285 41549..41899 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM372_RS00290 41949..42311 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / catA1 - 1..146168 146168


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T77694 WP_003026803.1 NZ_CP021856:c38119-37637 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T77694 NZ_CP021856:c38119-37637 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT77694 WP_003026799.1 NZ_CP021856:c38373-38107 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT77694 NZ_CP021856:c38373-38107 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References